Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase (BcCITase) mainly produces cycloisomaltooctaose. By structure-based mutagenesis of a carbohydrate-binding module family 35 (BcCBM35) and catalytic domain, the product specificity was modified and catalytic activity was improved, which enabled us to obtain a greater yield of cycloisomaltodecaose.

Keywords:
Bacillus circulans, cycloisomaltomegalosaccharide, cycloisomaltooligosaccharide, cycloisomaltooligosaccharide glucanotransferase, family 35 carbohydrate-binding module, subsite engineering
© The Authors Journal compilation © 2015 Biochemical Society
2015
You do not currently have access to this content.