We report that Tribbles 2 (TRB2), a pseudokinase linked to cancer, binds ATP and autophosphorylates in a metal-independent manner. This activity is prevented by mutations in the ATP-binding site and a TRB2 mutant can be targeted with a small molecule ligand.

Keywords:
adenosine 5′-triphosphate (ATP), chemical biology, kinase, pseudokinase, Tribbles, Tribbles 2 (TRB2/TRIB2), thermo-stability assay (TSA)
© The Authors Journal compilation © 2015 Biochemical Society
2015
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