On solid substrates, yeast colonies pass through distinct developmental phases characterized by the changes in pH of their surroundings from acidic to nearly alkaline and vice versa. At the beginning of the alkali phase colonies start to produce ammonia, which functions as a quorum-sensing molecule inducing the reprogramming of cell metabolism. Such reprogramming includes, among others, the activation of several plasma membrane transporters and is connected with colony differentiation. In the present study, we show that colony cells can use two transport mechanisms to import lactic acid: a ‘saturable’ component of the transport, which requires the presence of a functional Jen1p transporter, and a ‘non-saturable’ component (diffusion) that is independent of Jen1p. During colony development, the efficiency of both transport components changes similarly in central and outer colonial cells. Although the lactate uptake capacity of central cells gradually decreases during colony development, the lactate uptake capacity of outer cells peaks during the alkali phase and is also kept relatively high in the second acidic phase. This lactate uptake profile correlates with the localization of the Jen1p transporter to the plasma membrane of colony cells. Both lactic acid uptake mechanisms are diminished in sok2 colonies where JEN1 expression is decreased. The Sok2p transcription factor may therefore be involved in the regulation of non-saturable lactic acid uptake in yeast colonies.
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Research Article|
August 29 2013
The transport of carboxylic acids and important role of the Jen1p transporter during the development of yeast colonies
Sandra Paiva;
Sandra Paiva
1
*Molecular and Environmental Biology Centre (CBMA), Department of Biology, Campus de Gualtar, University of Minho, 4710-057 Braga, Portugal
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Dita Strachotová;
Dita Strachotová
1
†Institute of Microbiology of the AS CR, v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic
‡Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Viničná 5, 128 44 Prague 2, Czech Republic
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Helena Kučerová;
Helena Kučerová
†Institute of Microbiology of the AS CR, v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic
‡Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Viničná 5, 128 44 Prague 2, Czech Republic
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Otakar Hlaváček;
Otakar Hlaváček
†Institute of Microbiology of the AS CR, v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic
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Sandra Mota;
Sandra Mota
*Molecular and Environmental Biology Centre (CBMA), Department of Biology, Campus de Gualtar, University of Minho, 4710-057 Braga, Portugal
§Centre of Health and Environmental Research CISA, Superior School of Health Technology of Porto, Polytechnic Institute of Porto, 4200 Porto, Portugal
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Margarida Casal;
Margarida Casal
2
*Molecular and Environmental Biology Centre (CBMA), Department of Biology, Campus de Gualtar, University of Minho, 4710-057 Braga, Portugal
2Correspondence may be addressed to these authors (email vachova@biomed.cas.cz, zdenap@natur.cuni.cz or mcasal@bio.uminho.pt).
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Zdena Palková;
Zdena Palková
2
‡Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Viničná 5, 128 44 Prague 2, Czech Republic
2Correspondence may be addressed to these authors (email vachova@biomed.cas.cz, zdenap@natur.cuni.cz or mcasal@bio.uminho.pt).
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Libuše Váchová
Libuše Váchová
2
†Institute of Microbiology of the AS CR, v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic
2Correspondence may be addressed to these authors (email vachova@biomed.cas.cz, zdenap@natur.cuni.cz or mcasal@bio.uminho.pt).
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Publisher: Portland Press Ltd
Received:
February 20 2012
Revision Received:
June 12 2013
Accepted:
June 21 2013
Accepted Manuscript online:
June 21 2013
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2013 Biochemical Society
2013
Biochem J (2013) 454 (3): 551–558.
Article history
Received:
February 20 2012
Revision Received:
June 12 2013
Accepted:
June 21 2013
Accepted Manuscript online:
June 21 2013
Citation
Sandra Paiva, Dita Strachotová, Helena Kučerová, Otakar Hlaváček, Sandra Mota, Margarida Casal, Zdena Palková, Libuše Váchová; The transport of carboxylic acids and important role of the Jen1p transporter during the development of yeast colonies. Biochem J 15 September 2013; 454 (3): 551–558. doi: https://doi.org/10.1042/BJ20120312
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