Hv channels (voltage-gated proton channels) are expressed in blood cells, microglia and some types of epithelial cells. In neutrophils Hv channels regulate the production of reactive oxygen species through regulation of membrane potential and intracellular pH. Hv channels have also been suggested to play a role in sperm physiology in the human. However, the functions of the Hv channel at the whole-body level are not fully understood. In the present paper we show that Hvcn1 (voltage-gated hydrogen channel 1)-knockout mice show splenomegaly, autoantibodies and nephritis, that are reminiscent of human autoimmune diseases phenotypes. The number of activated T-cells was larger in Hvcn1-deficient mice than in the wild-type mice. Upon viral infection this was remarkably enhanced in Hvcn1-deficient mice. The production of superoxide anion in T-cells upon stimulation with PMA was significantly attenuated in the Hvcn1-deficient mice. These results suggest that Hv channels regulate T-cell homoeostasis in vivo.
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Research Article|
February 15 2013
Autoimmune disorder phenotypes in Hvcn1-deficient mice
Mari Sasaki;
Mari Sasaki
*Laboratory of Integrative Physiology, Department of Physiology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
†Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, Japan
‡Department of Molecular Medicine for Pathogenesis, Graduate School of Medicine, Ehime University, Toon, Ehime, Japan
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Akihiro Tojo;
Akihiro Tojo
§Department of Nephrology and Endocrinology, University of Tokyo, Tokyo, Japan
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Yoshifumi Okochi;
Yoshifumi Okochi
*Laboratory of Integrative Physiology, Department of Physiology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
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Nana Miyawaki;
Nana Miyawaki
*Laboratory of Integrative Physiology, Department of Physiology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
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Daisuke Kamimura;
Daisuke Kamimura
¶Laboratory of Developmental Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
∥Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan
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Akihito Yamaguchi;
Akihito Yamaguchi
†Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, Japan
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Masaaki Murakami;
Masaaki Murakami
¶Laboratory of Developmental Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
∥Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan
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Yasushi Okamura
Yasushi Okamura
1
*Laboratory of Integrative Physiology, Department of Physiology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
∥Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan
1To whom correspondence should be addressed (email yokamura@phys2.med.osaka-u.ac.jp).
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Publisher: Portland Press Ltd
Received:
August 29 2012
Revision Received:
December 07 2012
Accepted:
December 12 2012
Accepted Manuscript online:
December 12 2012
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2013 Biochemical Society
2013
Biochem J (2013) 450 (2): 295–301.
Article history
Received:
August 29 2012
Revision Received:
December 07 2012
Accepted:
December 12 2012
Accepted Manuscript online:
December 12 2012
Citation
Mari Sasaki, Akihiro Tojo, Yoshifumi Okochi, Nana Miyawaki, Daisuke Kamimura, Akihito Yamaguchi, Masaaki Murakami, Yasushi Okamura; Autoimmune disorder phenotypes in Hvcn1-deficient mice. Biochem J 1 March 2013; 450 (2): 295–301. doi: https://doi.org/10.1042/BJ20121188
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