NUPR1 (nuclear protein 1), also called P8 (molecular mass 8 kDa) or COM1 (candidate of metastasis 1), is involved in the stress response and in cancer progression. In the present study, we investigated whether human NUPR1 expression was regulated by TGFβ (transforming growth factor β), a secreted polypeptide largely involved in tumorigenesis. We demonstrate that the expression of NUPR1 was activated by TGFβ at the transcriptional level. We show that this activation is mediated by the SMAD proteins, which are transcription factors specifically involved in the signalling of TGFβ superfamily members. NUPR1 promoter analysis reveals the presence of a functional TGFβ-response element binding the SMAD proteins located in the genomic DNA region corresponding to the 5′-UTR (5′-untranslated region). Altogether, the molecular results of the present study, which demonstrate the existence of a TGFβ/SMAD/NUPR1 activation cascade, open the way to consider and investigate further a new mechanism enabling TGFβ to promote tumorigenesis by inducing stress resistance.
Skip Nav Destination
Article navigation
July 2012
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
Research Article|
June 27 2012
The human NUPR1/P8 gene is transcriptionally activated by transforming growth factor β via the SMAD signalling pathway
Roxane M. Pommier;
Roxane M. Pommier
1
*Inserm U1052, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
†CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
‡Université de Lyon, F-69000 Lyon, France
§Université Lyon 1, F-69000 Lyon, France
‖Centre Léon Bérard, F-69000 Lyon, France
Search for other works by this author on:
Johann Gout;
Johann Gout
1
*Inserm U1052, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
†CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
‡Université de Lyon, F-69000 Lyon, France
§Université Lyon 1, F-69000 Lyon, France
‖Centre Léon Bérard, F-69000 Lyon, France
Search for other works by this author on:
David F. Vincent;
David F. Vincent
*Inserm U1052, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
†CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
‡Université de Lyon, F-69000 Lyon, France
§Université Lyon 1, F-69000 Lyon, France
‖Centre Léon Bérard, F-69000 Lyon, France
Search for other works by this author on:
Carla E. Cano;
Carla E. Cano
¶Inserm U624, Stress Cellulaire, Parc Scientifique et Technologique de Luminy, F-13228 cedex 09 Marseille, France
Search for other works by this author on:
Bastien Kaniewski;
Bastien Kaniewski
*Inserm U1052, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
†CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
‡Université de Lyon, F-69000 Lyon, France
§Université Lyon 1, F-69000 Lyon, France
‖Centre Léon Bérard, F-69000 Lyon, France
Search for other works by this author on:
Sylvie Martel;
Sylvie Martel
*Inserm U1052, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
†CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
‡Université de Lyon, F-69000 Lyon, France
§Université Lyon 1, F-69000 Lyon, France
‖Centre Léon Bérard, F-69000 Lyon, France
Search for other works by this author on:
Jonathan Rodriguez;
Jonathan Rodriguez
*Inserm U1052, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
†CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
‡Université de Lyon, F-69000 Lyon, France
§Université Lyon 1, F-69000 Lyon, France
‖Centre Léon Bérard, F-69000 Lyon, France
Search for other works by this author on:
Geneviève Fourel;
Geneviève Fourel
*Inserm U1052, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
†CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
‡Université de Lyon, F-69000 Lyon, France
§Université Lyon 1, F-69000 Lyon, France
‖Centre Léon Bérard, F-69000 Lyon, France
Search for other works by this author on:
Ulrich Valcourt;
Ulrich Valcourt
*Inserm U1052, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
†CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
‡Université de Lyon, F-69000 Lyon, France
§Université Lyon 1, F-69000 Lyon, France
‖Centre Léon Bérard, F-69000 Lyon, France
Search for other works by this author on:
Julien C. Marie;
Julien C. Marie
*Inserm U1052, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
†CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
‡Université de Lyon, F-69000 Lyon, France
§Université Lyon 1, F-69000 Lyon, France
‖Centre Léon Bérard, F-69000 Lyon, France
Search for other works by this author on:
Juan L. Iovanna;
Juan L. Iovanna
¶Inserm U624, Stress Cellulaire, Parc Scientifique et Technologique de Luminy, F-13228 cedex 09 Marseille, France
Search for other works by this author on:
Laurent Bartholin
Laurent Bartholin
2
*Inserm U1052, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
†CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, F-69008 Lyon, France
‡Université de Lyon, F-69000 Lyon, France
§Université Lyon 1, F-69000 Lyon, France
‖Centre Léon Bérard, F-69000 Lyon, France
2To whom correspondence should be addressed (email laurent.bartholin@lyon.unicancer.fr).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
March 05 2012
Revision Received:
May 01 2012
Accepted:
May 01 2012
Accepted Manuscript online:
June 27 2012
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2012 Biochemical Society
2012
Biochem J (2012) 445 (2): 285–293.
Article history
Received:
March 05 2012
Revision Received:
May 01 2012
Accepted:
May 01 2012
Accepted Manuscript online:
June 27 2012
Citation
Roxane M. Pommier, Johann Gout, David F. Vincent, Carla E. Cano, Bastien Kaniewski, Sylvie Martel, Jonathan Rodriguez, Geneviève Fourel, Ulrich Valcourt, Julien C. Marie, Juan L. Iovanna, Laurent Bartholin; The human NUPR1/P8 gene is transcriptionally activated by transforming growth factor β via the SMAD signalling pathway. Biochem J 15 July 2012; 445 (2): 285–293. doi: https://doi.org/10.1042/BJ20120368
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.