Although many cancer vaccines have been developed against type I MAGE (melanoma antigen) genes owing to their shared tumour-specific expression properties, studies about their expression and functions are relatively limited. In the present study, we first identify a non-testis-specific type I MAGE gene, Mageb18 (melanoma antigen family B 18). Mouse Mageb18 is also expressed in digestion- and immune-related tissues as well as testis, and its expression in testis is age-dependent. Mageb18 is expressed in many mouse-derived cell lines, and DNA demethylation and histone acetylation mediate the reactivation of Mageb18 in Mageb18-negtive H22 and C6 cells. We also show that mouse Mageb18 encodes a 46 kDa protein which is predominantly localized in the cytoplasm. In testis, the endogenous MAGEB18 protein is mainly expressed in proliferative spermatogonia and primary and secondary spermatocytes, but less so in spermatids. Finally, we demonstrate that knockdown of MAGEB18 inhibits the growth of B16-F0 cells and induces apoptosis, which correlates with increased levels of TP53 (tumour protein 53), p21, Bax and caspase 3. The results of the present study thus uncover an important phenomenon that the expression of certain type I MAGE genes, at least for Mageb18, is non-testis-specific. Although they can regulate various malignant phenotypes of cancer cells, it is necessary to study further their expression pattern in normal tissues before using them to develop more effective and safer cancer vaccines.
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Research Article|
April 16 2012
The mouse Mageb18 gene encodes a ubiquitously expressed type I MAGE protein and regulates cell proliferation and apoptosis in melanoma B16-F0 cells
Yuquan Lin;
Yuquan Lin
1The State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, People's Republic of China
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Ti Wen;
Ti Wen
1The State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, People's Republic of China
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Xianyi Meng;
Xianyi Meng
1The State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, People's Republic of China
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Zhenzhou Wu;
Zhenzhou Wu
1
1The State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, People's Republic of China
1Correspondence may be addressed to either of these authors (email naturepower@nankai.edu.cn or yinzn@nankai.edu.cn).
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Liqing Zhao;
Liqing Zhao
1The State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, People's Republic of China
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Puyue Wang;
Puyue Wang
1The State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, People's Republic of China
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Zhangyong Hong;
Zhangyong Hong
1The State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, People's Republic of China
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Zhinan Yin
Zhinan Yin
1
1The State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, People's Republic of China
1Correspondence may be addressed to either of these authors (email naturepower@nankai.edu.cn or yinzn@nankai.edu.cn).
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Publisher: Portland Press Ltd
Received:
November 29 2011
Revision Received:
February 11 2012
Accepted:
February 15 2012
Accepted Manuscript online:
February 15 2012
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2012 Biochemical Society
2012
Biochem J (2012) 443 (3): 779–788.
Article history
Received:
November 29 2011
Revision Received:
February 11 2012
Accepted:
February 15 2012
Accepted Manuscript online:
February 15 2012
Citation
Yuquan Lin, Ti Wen, Xianyi Meng, Zhenzhou Wu, Liqing Zhao, Puyue Wang, Zhangyong Hong, Zhinan Yin; The mouse Mageb18 gene encodes a ubiquitously expressed type I MAGE protein and regulates cell proliferation and apoptosis in melanoma B16-F0 cells. Biochem J 1 May 2012; 443 (3): 779–788. doi: https://doi.org/10.1042/BJ20112054
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