Agam (Anopheles gambiae) relies on its olfactory system to target human prey, leading eventually to the injection of Plasmodium falciparum, the malaria vector. OBPs (odorant-binding proteins) are the first line of proteins involved in odorant recognition. They interact with olfactory receptors and thus constitute an interesting target for insect control. In the present study, we undertook a large-scale analysis of proteins belonging to the olfactory system of Agam with the aim of preventing insect bites by designing strong olfactory repellents. We determined the three-dimensional structures of several Agam OBPs, either alone or in complex with model compounds. In the present paper, we report the first three-dimensional structure of a member of the C-plus class of OBPs, AgamOBP47, which has a longer sequence than classical OBPs and contains six disulfide bridges. AgamOBP47 possesses a core of six α-helices and three disulfide bridges, similar to the classical OBP fold. Two extra loops and the N- and C-terminal extra segments contain two additional α-helices and are held in conformation by three disulfide bridges. They are located either side of the classical OBP core domain. The binding site of OBP47 is located between the core and the additional domains. Two crevices are observed on opposite sides of OBP47, which are joined together by a shallow channel of sufficient size to accommodate a model of the best-tested ligand. The binding sites of C-plus class OBPs therefore exhibit different characteristics, as compared with classical OBPs, which should lead to markedly diverse functional implications.
Skip Nav Destination
Article navigation
August 2011
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
Research Article|
July 13 2011
Crystal structure of a novel type of odorant-binding protein from Anopheles gambiae, belonging to the C-plus class
Amandine Lagarde;
Amandine Lagarde
*Architecture et Fonction des Macromolécules Biologiques, UMR 6098 CNRS and University of Marseille, 163 Av. de Luminy Case 932, 13288 Marseille Cedex 09, France
Search for other works by this author on:
Silvia Spinelli;
Silvia Spinelli
*Architecture et Fonction des Macromolécules Biologiques, UMR 6098 CNRS and University of Marseille, 163 Av. de Luminy Case 932, 13288 Marseille Cedex 09, France
Search for other works by this author on:
Huili Qiao;
Huili Qiao
†Department of Biology of Agricultural Plants, University of Pisa, via S. Michele, 4, 56124 Pisa, Italy
Search for other works by this author on:
Mariella Tegoni;
Mariella Tegoni
*Architecture et Fonction des Macromolécules Biologiques, UMR 6098 CNRS and University of Marseille, 163 Av. de Luminy Case 932, 13288 Marseille Cedex 09, France
Search for other works by this author on:
Paolo Pelosi;
Paolo Pelosi
†Department of Biology of Agricultural Plants, University of Pisa, via S. Michele, 4, 56124 Pisa, Italy
Search for other works by this author on:
Christian Cambillau
Christian Cambillau
1
*Architecture et Fonction des Macromolécules Biologiques, UMR 6098 CNRS and University of Marseille, 163 Av. de Luminy Case 932, 13288 Marseille Cedex 09, France
1To whom correspondence should be addressed (email cambillau@afmb.univ-mrs.fr).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
March 22 2011
Revision Received:
May 11 2011
Accepted:
May 12 2011
Accepted Manuscript online:
May 12 2011
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biochem J (2011) 437 (3): 423–430.
Article history
Received:
March 22 2011
Revision Received:
May 11 2011
Accepted:
May 12 2011
Accepted Manuscript online:
May 12 2011
Citation
Amandine Lagarde, Silvia Spinelli, Huili Qiao, Mariella Tegoni, Paolo Pelosi, Christian Cambillau; Crystal structure of a novel type of odorant-binding protein from Anopheles gambiae, belonging to the C-plus class. Biochem J 1 August 2011; 437 (3): 423–430. doi: https://doi.org/10.1042/BJ20110522
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.