RNF4 [RING (really interesting new gene) finger protein 4] family ubiquitin ligases are RING E3 ligases that regulate the homoeostasis of SUMOylated proteins by promoting their ubiquitylation. In the present paper we report that the RING domain of RNF4 forms a stable dimer, and that dimerization is required for ubiquitin transfer. Our results suggest that the stability of the E2~ubiquitin thioester bond is regulated by RING domain dimerization.

Keywords:
E3 ligase, protein domain, protein–protein interaction, really interesting new gene finger (RING finger), RING finger protein 4 (RNF4), small ubiquitin-like modifier (SUMO), ubiquitin
© The Authors Journal compilation © 2010 Biochemical Society
2010
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