When a plant cell is challenged by a well-defined stimulus, complex signal transduction pathways are activated to promote the modulation of specific sets of genes and eventually to develop adaptive responses. In this context, protein phosphorylation plays a fundamental role through the activation of multiple protein kinase families. Although the involvement of protein kinases at the plasma membrane and cytosolic levels are now well-documented, their nuclear counterparts are still poorly investigated. In the field of plant defence reactions, no known study has yet reported the activation of a nuclear protein kinase and/or its nuclear activity in plant cells, although some protein kinases, e.g. MAPK (mitogen-activated protein kinase), are known to be translocated into the nucleus. In the present study, we investigated the ability of cryptogein, a proteinaceous elicitor of tobacco defence reactions, to induce different nuclear protein kinase activities. We found that at least four nuclear protein kinases are activated in response to cryptogein treatment in a time-dependent manner, some of them exhibiting Ca2+-dependent activity. The present study focused on one 47 kDa protein kinase with a Ca2+-independent activity, closely related to the MAPK family. After purification and microsequencing, this protein kinase was formally identified as SIPK (salicyclic acid-induced protein kinase), a biotic and abiotic stress-activated MAPK of tobacco. We also showed that cytosolic activation of SIPK is not sufficient to promote a nuclear SIPK activity, the latter being correlated with cell death. In that way, the present study provides evidence of a functional nuclear MAPK activity involved in response to an elicitor treatment.
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February 2009
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Research Article|
January 28 2009
Activation of a nuclear-localized SIPK in tobacco cells challenged by cryptogein, an elicitor of plant defence reactions
Jennifer Dahan;
Jennifer Dahan
*UMR INRA 1088/CNRS 5184/Université de Bourgogne Plante-Microbe-Environnement, 17 Rue Sully, BP 86510, 21065 Dijon cédex, France
†GDR CNRS N°2688, Calcium et régulation de l'expression des gènes en contexte normal et pathologique, 118 route de Narbonne, 31062 Toulouse cedex, France
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Carole Pichereaux;
Carole Pichereaux
‡Plateforme de protéomique Toulouse Midi-Pyrénées, IPBS/IFR40, 205 route de Narbonne, 31077 Toulouse, France
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Michel Rossignol;
Michel Rossignol
‡Plateforme de protéomique Toulouse Midi-Pyrénées, IPBS/IFR40, 205 route de Narbonne, 31077 Toulouse, France
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Sabrina Blanc;
Sabrina Blanc
*UMR INRA 1088/CNRS 5184/Université de Bourgogne Plante-Microbe-Environnement, 17 Rue Sully, BP 86510, 21065 Dijon cédex, France
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David Wendehenne;
David Wendehenne
*UMR INRA 1088/CNRS 5184/Université de Bourgogne Plante-Microbe-Environnement, 17 Rue Sully, BP 86510, 21065 Dijon cédex, France
†GDR CNRS N°2688, Calcium et régulation de l'expression des gènes en contexte normal et pathologique, 118 route de Narbonne, 31062 Toulouse cedex, France
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Alain Pugin;
Alain Pugin
*UMR INRA 1088/CNRS 5184/Université de Bourgogne Plante-Microbe-Environnement, 17 Rue Sully, BP 86510, 21065 Dijon cédex, France
†GDR CNRS N°2688, Calcium et régulation de l'expression des gènes en contexte normal et pathologique, 118 route de Narbonne, 31062 Toulouse cedex, France
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Stéphane Bourque
Stéphane Bourque
1
*UMR INRA 1088/CNRS 5184/Université de Bourgogne Plante-Microbe-Environnement, 17 Rue Sully, BP 86510, 21065 Dijon cédex, France
†GDR CNRS N°2688, Calcium et régulation de l'expression des gènes en contexte normal et pathologique, 118 route de Narbonne, 31062 Toulouse cedex, France
1To whom correspondence should be addressed (email bourque@dijon.inra.fr).
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Publisher: Portland Press Ltd
Received:
July 22 2008
Revision Received:
October 07 2008
Accepted:
October 16 2008
Accepted Manuscript online:
October 16 2008
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2009 Biochemical Society
2009
Biochem J (2009) 418 (1): 191–200.
Article history
Received:
July 22 2008
Revision Received:
October 07 2008
Accepted:
October 16 2008
Accepted Manuscript online:
October 16 2008
Citation
Jennifer Dahan, Carole Pichereaux, Michel Rossignol, Sabrina Blanc, David Wendehenne, Alain Pugin, Stéphane Bourque; Activation of a nuclear-localized SIPK in tobacco cells challenged by cryptogein, an elicitor of plant defence reactions. Biochem J 15 February 2009; 418 (1): 191–200. doi: https://doi.org/10.1042/BJ20081465
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