Light signal transduction in plants involves an intricate series of pathways which is finely regulated by interactions between specific signalling proteins, as well as by protein modifications such as phosphorylation and ubiquitination. The identification of novel phytochrome-interacting proteins and the precise signalling mechanisms that they mediate is still ongoing. In our present study, we show that the newly identified putative phytochrome-associated protein, PAPP2C (phytochrome-associated protein phosphatase type 2C), interacts in the nucleus with phyA (phytochrome A) and phyB, both in vitro and in vivo. Moreover, the phosphatase activity of PAPP2C and its association with phytochromes were found to be enhanced by red light, indicating that it plays a role in mediating phytochrome signalling. In particular, PAPP2C specifically binds to the N-terminal PHY domain of the phytochromes. We thus speculate that this interaction reflects a unique regulatory function of this phosphatase toward established phytochrome-associated proteins. We also show that PAPP2C effectively dephosphorylates phytochromes in vitro. Interestingly, PAPP2C indirectly mediates the dephosphorylation of PIF3 (phytochrome-interacting factor 3) in vitro. Taken together, we suggest that PAPP2C functions as a regulator of PIF3 by dephosphorylating phytochromes in the nucleus.
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Research Article|
September 25 2008
A novel protein phosphatase indirectly regulates phytochrome-interacting factor 3 via phytochrome
Bong-Kwan Phee;
Bong-Kwan Phee
1
*Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University, Yongin, Korea, 446-701
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Jeong-Il Kim;
Jeong-Il Kim
1
†Department of Molecular Biotechnology and Kumho Life Science Laboratory, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea, 500-757
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Dong Ho Shin;
Dong Ho Shin
*Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University, Yongin, Korea, 446-701
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Jihye Yoo;
Jihye Yoo
*Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University, Yongin, Korea, 446-701
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Kyoung-Jin Park;
Kyoung-Jin Park
†Department of Molecular Biotechnology and Kumho Life Science Laboratory, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea, 500-757
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Yun-Jeong Han;
Yun-Jeong Han
†Department of Molecular Biotechnology and Kumho Life Science Laboratory, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea, 500-757
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Yong-Kook Kwon;
Yong-Kook Kwon
*Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University, Yongin, Korea, 446-701
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Man-Ho Cho;
Man-Ho Cho
*Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University, Yongin, Korea, 446-701
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Jong-Seong Jeon;
Jong-Seong Jeon
*Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University, Yongin, Korea, 446-701
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Seong Hee Bhoo;
Seong Hee Bhoo
2
*Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University, Yongin, Korea, 446-701
2Correspondence may be addressed to either of these authors (email trhahn@khu.ac.kr or shbhoo@khu.ac.kr).
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Tae-Ryong Hahn
Tae-Ryong Hahn
2
*Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University, Yongin, Korea, 446-701
2Correspondence may be addressed to either of these authors (email trhahn@khu.ac.kr or shbhoo@khu.ac.kr).
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Publisher: Portland Press Ltd
Received:
November 14 2007
Revision Received:
May 29 2008
Accepted:
June 20 2008
Accepted Manuscript online:
June 20 2008
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biochem J (2008) 415 (2): 247–255.
Article history
Received:
November 14 2007
Revision Received:
May 29 2008
Accepted:
June 20 2008
Accepted Manuscript online:
June 20 2008
Citation
Bong-Kwan Phee, Jeong-Il Kim, Dong Ho Shin, Jihye Yoo, Kyoung-Jin Park, Yun-Jeong Han, Yong-Kook Kwon, Man-Ho Cho, Jong-Seong Jeon, Seong Hee Bhoo, Tae-Ryong Hahn; A novel protein phosphatase indirectly regulates phytochrome-interacting factor 3 via phytochrome. Biochem J 15 October 2008; 415 (2): 247–255. doi: https://doi.org/10.1042/BJ20071555
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