DNA topoisomerase I together with the other cellular DNA topoisomerases releases the torsional stress from DNA caused by processes such as replication, transcription and recombination. Despite the well-defined knowledge of its mechanism of action, DNA topoisomerase I in vivo activity has been only partially characterized. In fact the basic question concerning the capability of the enzyme to cleave and rejoin DNA wrapped around a histone octamer remains still unanswered. By studying both in vivo and in vitro the cleavage activity of DNA topoisomerase I in the presence of camptothecin on a repeated trinucleotide sequence, (TTA)35, lying in chromosome XIII of Saccharomyces cerevisiae, we can conclude that nucleosomes represent a physical barrier for the enzyme activity.
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February 2008
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Research Article|
January 15 2008
Nucleosomes represent a physical barrier for cleavage activity of DNA topoisomerase I in vivo
Francesca Di Felice;
Francesca Di Felice
*Dipartimento di Genetica e Biologia Molecolare Università di Roma La Sapienza, Roma, 00185 Italy
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Francesco Chiani;
Francesco Chiani
*Dipartimento di Genetica e Biologia Molecolare Università di Roma La Sapienza, Roma, 00185 Italy
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Giorgio Camilloni
Giorgio Camilloni
1
*Dipartimento di Genetica e Biologia Molecolare Università di Roma La Sapienza, Roma, 00185 Italy
†Istituto Biologia e Patologia Molecolari, CNR, Roma, 00185 Italy
1To whom correspondence should be addressed (email giorgio.camilloni@uniroma1.it).
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Publisher: Portland Press Ltd
Received:
July 06 2007
Revision Received:
October 18 2007
Accepted:
October 30 2007
Accepted Manuscript online:
October 30 2007
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biochem J (2008) 409 (3): 651–656.
Article history
Received:
July 06 2007
Revision Received:
October 18 2007
Accepted:
October 30 2007
Accepted Manuscript online:
October 30 2007
Citation
Francesca Di Felice, Francesco Chiani, Giorgio Camilloni; Nucleosomes represent a physical barrier for cleavage activity of DNA topoisomerase I in vivo. Biochem J 1 February 2008; 409 (3): 651–656. doi: https://doi.org/10.1042/BJ20070893
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