DGKγ (diacylglycerol kinase γ) was reported to interact with β2-chimaerin, a GAP (GTPase-activating protein) for Rac, in response to epidermal growth factor. Here we found that PMA and H2O2 also induced the interaction of DGKγ with β2-chimaerin. It is noteworthy that simultaneous addition of PMA and H2O2 synergistically enhanced the interaction. In this case, PMA was replaceable by DAG (diacylglycerol). The β2-chimaerin translocation from the cytoplasm to the plasma membrane caused by PMA plus H2O2 was further enhanced by the expression of DGKγ. Moreover, DGKγ apparently enhanced the β2-chimaerin GAP activity upon cell stimulation with PMA. PMA was found to be mainly required for a conversion of β2-chimaerin into an active form. On the other hand, H2O2 was suggested to induce a release of Zn2+ from the C1 domain of β2-chimaerin. By stepwise deletion analysis, we demonstrated that the SH2 (Src homology 2) and C1 domains of β2-chimaerin interacted with the N-terminal half of catalytic region of DGKγ. Unexpectedly, the SH2 domain of β2-chimaerin contributes to the interaction independently of phosphotyrosine. Taken together, these results suggest that the functional link between DGKγ and β2-chimaerin has a broad significance in response to a wide range of cell stimuli. Our work offers a novel mechanism of protein–protein interaction, that is, the phosphotyrosine-independent interaction of the SH2 domain acting in co-operation with the C1 domain.
Skip Nav Destination
Article navigation
January 2008
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
Research Article|
December 11 2007
Phorbol ester and hydrogen peroxide synergistically induce the interaction of diacylglycerol kinase γ with the Src homology 2 and C1 domains of β2-chimaerin
Satoshi Yasuda;
Satoshi Yasuda
1Department of Biochemistry, Sapporo Medical University School of Medicine, South-1, West-17, Chuo-ku, Sapporo, 060-8556, Japan
Search for other works by this author on:
Masahiro Kai;
Masahiro Kai
1Department of Biochemistry, Sapporo Medical University School of Medicine, South-1, West-17, Chuo-ku, Sapporo, 060-8556, Japan
Search for other works by this author on:
Shin-ichi Imai;
Shin-ichi Imai
1Department of Biochemistry, Sapporo Medical University School of Medicine, South-1, West-17, Chuo-ku, Sapporo, 060-8556, Japan
Search for other works by this author on:
Hideo Kanoh;
Hideo Kanoh
1Department of Biochemistry, Sapporo Medical University School of Medicine, South-1, West-17, Chuo-ku, Sapporo, 060-8556, Japan
Search for other works by this author on:
Fumio Sakane
Fumio Sakane
1
1Department of Biochemistry, Sapporo Medical University School of Medicine, South-1, West-17, Chuo-ku, Sapporo, 060-8556, Japan
1To whom correspondence should be addessed (email sakane@sapmed.ac.jp).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
June 27 2007
Revision Received:
August 28 2007
Accepted:
September 06 2007
Accepted Manuscript online:
September 06 2007
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biochem J (2008) 409 (1): 95–106.
Article history
Received:
June 27 2007
Revision Received:
August 28 2007
Accepted:
September 06 2007
Accepted Manuscript online:
September 06 2007
Citation
Satoshi Yasuda, Masahiro Kai, Shin-ichi Imai, Hideo Kanoh, Fumio Sakane; Phorbol ester and hydrogen peroxide synergistically induce the interaction of diacylglycerol kinase γ with the Src homology 2 and C1 domains of β2-chimaerin. Biochem J 1 January 2008; 409 (1): 95–106. doi: https://doi.org/10.1042/BJ20070848
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.