The yeast Saccharomyces cerevisiae is a facultative anaerobic organism. Under anaerobiosis, sustained growth relies on the presence of exogenously supplied unsaturated fatty acids and ergosterol that yeast is unable to synthesize in the absence of oxygen or upon haem depletion. In the absence of exogenous supplementation with unsaturated fatty acid, a net accumulation of SFA (saturated fatty acid) is observed that induces significant modification of phospholipid profile [Ferreira, Régnacq, Alimardani, Moreau-Vauzelle and Bergès (2004) Biochem. J. 378, 899–908]. In the present paper, we focus on the role of SFH2/CSR1, a hypoxic gene related to SEC14 and its involvement in lipid metabolism upon haem depletion in the absence of oleic acid supplementation. We observed that inactivation of SFH2 results in enhanced accumulation of SFA and phospholipid metabolism alterations. It results in premature growth arrest and leads to an exacerbated sensitivity to exogenous SFA. This phenotype is suppressed in the presence of exogenous oleic acid, or by a controlled expression of FAS1, one of the two genes encoding FAS. We present several lines of evidence to suggest that Sfh2p and oleic acid regulate SFA synthase in yeast at different levels: whereas oleic acid acts on FAS2 at the transcriptional level, we show that Sfh2p inhibits fatty acid synthase activity in response to haem depletion.
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Research Article|
December 11 2007
SFH2 regulates fatty acid synthase activity in the yeast Saccharomyces cerevisiae and is critical to prevent saturated fatty acid accumulation in response to haem and oleic acid depletion
Thomas Desfougères;
Thomas Desfougères
1Laboratoire de Génétique de la Levure CNRS-UMR6161, Université de Poitiers, 40 avenue du Recteur Pineau, 86022 Poitiers cedex, France
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Thierry Ferreira;
Thierry Ferreira
1Laboratoire de Génétique de la Levure CNRS-UMR6161, Université de Poitiers, 40 avenue du Recteur Pineau, 86022 Poitiers cedex, France
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Thierry Bergès;
Thierry Bergès
1Laboratoire de Génétique de la Levure CNRS-UMR6161, Université de Poitiers, 40 avenue du Recteur Pineau, 86022 Poitiers cedex, France
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Matthieu Régnacq
Matthieu Régnacq
1
1Laboratoire de Génétique de la Levure CNRS-UMR6161, Université de Poitiers, 40 avenue du Recteur Pineau, 86022 Poitiers cedex, France
1To whom correspondance should be addressed (email matthieu.regnacq@univ-poitiers.fr).
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Publisher: Portland Press Ltd
Received:
July 30 2007
Revision Received:
August 30 2007
Accepted:
September 06 2007
Accepted Manuscript online:
September 06 2007
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biochem J (2008) 409 (1): 299–309.
Article history
Received:
July 30 2007
Revision Received:
August 30 2007
Accepted:
September 06 2007
Accepted Manuscript online:
September 06 2007
Citation
Thomas Desfougères, Thierry Ferreira, Thierry Bergès, Matthieu Régnacq; SFH2 regulates fatty acid synthase activity in the yeast Saccharomyces cerevisiae and is critical to prevent saturated fatty acid accumulation in response to haem and oleic acid depletion. Biochem J 1 January 2008; 409 (1): 299–309. doi: https://doi.org/10.1042/BJ20071028
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