Hormone-refractory prostate carcinomas as well as the neovasculature of different tumours express high levels of PSMA (prostate-specific membrane antigen). PSMA is a type II-transmembrane glycoprotein and a potential tumour marker for both diagnosis and passive immunotherapy. Here, we report on the association of PSMA with DRMs (detergent-resistant membranes) at different stages of the protein maturation pathway in human prostate carcinoma LNCaP cells. At least three PSMA glycoforms were biochemically identified based on their extractability behaviour in different non-ionic detergents. In particular, one precursor glycoform of PSMA is associated with Tween 20-insoluble DRMs, whereas the complex glycosylated protein segregates into membrane structures that are insoluble in Lubrol WX and display a different lipid composition. Association of PSMA with these membranes occurs in the Golgi compartment together with the acquisition of a native conformation. PSMA homodimers reach the plasma membrane of LNCaP cells in Lubrol WX-insoluble lipid/protein complexes. At the steady state, the majority of PSMA remains within these membrane microdomains at the cell surface. We conclude that the intracellular transport of PSMA occurs through populations of DRMs distinct for each biosynthetic form and cellular compartment.
Skip Nav Destination
Article navigation
January 2008
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
Research Article|
December 11 2007
Different glycoforms of prostate-specific membrane antigen are intracellularly transported through their association with distinct detergent-resistant membranes
Deborah Castelletti;
Deborah Castelletti
1
*Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany
Search for other works by this author on:
Marwan Alfalah;
Marwan Alfalah
1
*Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany
Search for other works by this author on:
Martin Heine;
Martin Heine
1
*Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany
Search for other works by this author on:
Zeynep Hein;
Zeynep Hein
*Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany
Search for other works by this author on:
Ruth Schmitte;
Ruth Schmitte
*Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany
Search for other works by this author on:
Giulio Fracasso;
Giulio Fracasso
†Department of Pathology, University of Verona, Policlinico G.B. Rossi, P. le L.A. Scuro n.10, 37134 Italy
Search for other works by this author on:
Marco Colombatti;
Marco Colombatti
†Department of Pathology, University of Verona, Policlinico G.B. Rossi, P. le L.A. Scuro n.10, 37134 Italy
Search for other works by this author on:
Hassan Y. Naim
Hassan Y. Naim
2
†Department of Pathology, University of Verona, Policlinico G.B. Rossi, P. le L.A. Scuro n.10, 37134 Italy
2To whom correspondence should be addressed (email hassan.naim@tiho-hannover.de).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
March 22 2007
Revision Received:
October 11 2007
Accepted:
October 12 2007
Accepted Manuscript online:
October 12 2007
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biochem J (2008) 409 (1): 149–157.
Article history
Received:
March 22 2007
Revision Received:
October 11 2007
Accepted:
October 12 2007
Accepted Manuscript online:
October 12 2007
Citation
Deborah Castelletti, Marwan Alfalah, Martin Heine, Zeynep Hein, Ruth Schmitte, Giulio Fracasso, Marco Colombatti, Hassan Y. Naim; Different glycoforms of prostate-specific membrane antigen are intracellularly transported through their association with distinct detergent-resistant membranes. Biochem J 1 January 2008; 409 (1): 149–157. doi: https://doi.org/10.1042/BJ20070396
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.