Osterix, a zinc-finger-containing transcription factor, is required for osteoblast differentiation and bone formation. Osterix is also expressed in dental mesenchymal cells of the tooth germ. However, transcriptional regulation by Osterix in tooth development is not clear. Genetic studies in osteogenesis place Osterix downstream of Runx2 (Runt-related 2). The expression of Osterix in odontoblasts overlaps with Runx3 during terminal differentiation in vivo. Runx3 down-regulates Osterix expression in mouse DPCs (dental pulp cells). Therefore the regulatory role of Runx3 on Osterix expression in tooth development was investigated. Enforced expression of Runx3 down-regulated the activity of the Osterix promoter in the human embryonic kidney 293 cell line. When the Runx3 responsive element on the Osterix promoter, located at −713 to −707 bp (site 3, AGTGGTT) relative to the cap site, was mutated, this down-regulation was abrogated. Furthermore, electrophoretic mobility-shift assay and chromatin immunoprecipitation assays in mouse DPCs demonstrated direct functional binding of Runx3 to the Osterix promoter. These results demonstrate the transcriptional regulation of Osterix expression by Runx3 during differentiation of dental pulp cells into odontoblasts during tooth development.
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Research Article|
June 13 2007
Runx3 negatively regulates Osterix expression in dental pulp cells
Li Zheng;
Li Zheng
*Laboratory of Oral Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Aichi 474-8522, Japan
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Koichiro Iohara;
Koichiro Iohara
*Laboratory of Oral Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Aichi 474-8522, Japan
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Masaki Ishikawa;
Masaki Ishikawa
†Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan
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Takeshi Into;
Takeshi Into
*Laboratory of Oral Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Aichi 474-8522, Japan
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Teruko Takano-Yamamoto;
Teruko Takano-Yamamoto
‡Division of Orthodontics and Dentofacial Orthopedics, Graduate School of Dentistry, Tohoku University, Sendai 980-8575, Japan
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Kenji Matsushita;
Kenji Matsushita
*Laboratory of Oral Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Aichi 474-8522, Japan
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Misako Nakashima
Misako Nakashima
1
*Laboratory of Oral Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Aichi 474-8522, Japan
1To whom correspondence should be addressed (email misako@nils.go.jp).
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Publisher: Portland Press Ltd
Received:
January 18 2007
Revision Received:
February 19 2007
Accepted:
March 13 2007
Accepted Manuscript online:
March 13 2007
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2007 Biochemical Society
2007
Biochem J (2007) 405 (1): 69–75.
Article history
Received:
January 18 2007
Revision Received:
February 19 2007
Accepted:
March 13 2007
Accepted Manuscript online:
March 13 2007
Citation
Li Zheng, Koichiro Iohara, Masaki Ishikawa, Takeshi Into, Teruko Takano-Yamamoto, Kenji Matsushita, Misako Nakashima; Runx3 negatively regulates Osterix expression in dental pulp cells. Biochem J 1 July 2007; 405 (1): 69–75. doi: https://doi.org/10.1042/BJ20070104
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