Evolutionarily conserved SR proteins (serine/arginine-rich proteins) are important factors for alternative splicing and their activity is modulated by SRPKs (SR protein-specific kinases). We previously identified Dsk1p (dis1-suppressing protein kinase) as the orthologue of human SRPK1 in fission yeast. In addition to its similarity of gene structure to higher eukaryotes, fission yeast Schizosaccharomyces pombe is a unicellular eukaryotic organism in which alternative splicing takes place. In the present study, we have revealed for the first time that SR proteins, Srp1p and Srp2p, are the in vivo substrates of Dsk1p in S. pombe. Moreover, the cellular localization of the SR proteins and Prp2p splicing factor is dependent on dsk1+: Dsk1p is required for the efficient nuclear localization of Srp2p and Prp2p, while it promotes the cytoplasmic distribution of Srp1p, thereby differentially influencing the destinations of these proteins in the cell. The present study offers the first biochemical and genetic evidence for the in vivo targets of the SRPK1 orthologue, Dsk1p, in S. pombe and the significant correlation between Dsk1p-mediated phosphorylation and the cellular localization of the SR proteins, providing information about the physiological functions of Dsk1p. Furthermore, the results demonstrate that the regulatory function of SRPKs in the nuclear targeting of SR proteins is conserved from fission yeast to human, indicating a general mechanism of reversible phosphorylation to control the activities of SR proteins in RNA metabolism through cellular partitioning.
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Research Article|
June 13 2007
Dsk1p kinase phosphorylates SR proteins and regulates their cellular localization in fission yeast
Zhaohua Tang;
Zhaohua Tang
1
1W.M. Keck Science Center, 925 North Mills Avenue, The Claremont Colleges, Claremont, CA 91711, U.S.A.
1To whom correspondence should be addressed (email ztang@jsd.claremont.edu).
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Amy Tsurumi;
Amy Tsurumi
1W.M. Keck Science Center, 925 North Mills Avenue, The Claremont Colleges, Claremont, CA 91711, U.S.A.
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Sarah Alaei;
Sarah Alaei
1W.M. Keck Science Center, 925 North Mills Avenue, The Claremont Colleges, Claremont, CA 91711, U.S.A.
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Christopher Wilson;
Christopher Wilson
1W.M. Keck Science Center, 925 North Mills Avenue, The Claremont Colleges, Claremont, CA 91711, U.S.A.
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Cathleen Chiu;
Cathleen Chiu
1W.M. Keck Science Center, 925 North Mills Avenue, The Claremont Colleges, Claremont, CA 91711, U.S.A.
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Jessica Oya;
Jessica Oya
1W.M. Keck Science Center, 925 North Mills Avenue, The Claremont Colleges, Claremont, CA 91711, U.S.A.
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Benson Ngo
Benson Ngo
1W.M. Keck Science Center, 925 North Mills Avenue, The Claremont Colleges, Claremont, CA 91711, U.S.A.
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Publisher: Portland Press Ltd
Received:
October 09 2006
Revision Received:
February 28 2007
Accepted:
March 16 2007
Accepted Manuscript online:
March 16 2007
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2007 Biochemical Society
2007
Biochem J (2007) 405 (1): 21–30.
Article history
Received:
October 09 2006
Revision Received:
February 28 2007
Accepted:
March 16 2007
Accepted Manuscript online:
March 16 2007
Citation
Zhaohua Tang, Amy Tsurumi, Sarah Alaei, Christopher Wilson, Cathleen Chiu, Jessica Oya, Benson Ngo; Dsk1p kinase phosphorylates SR proteins and regulates their cellular localization in fission yeast. Biochem J 1 July 2007; 405 (1): 21–30. doi: https://doi.org/10.1042/BJ20061523
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