CUG-BP1 [CUG-binding protein 1 also called CELF (CUG-BP1 and ETR3 like factors) 1] is a human RNA-binding protein that has been implicated in the control of splicing and mRNA translation. The Xenopus homologue [EDEN-BP (embryo deadenylation element-binding protein)] is required for rapid deadenylation of certain maternal mRNAs just after fertilization. A variety of sequence elements have been described as target sites for these two proteins but their binding specificity is still controversial. Using a SELEX (systematic evolution of ligand by exponential enrichment) procedure and recombinant CUG-BP1 we selected two families of aptamers. Surface plasmon resonance and electrophoretic mobility-shift assays showed that these two families differed in their ability to bind CUG-BP1. Furthermore, the selected high-affinity aptamers form two complexes with CUG-BP1 in electrophoretic mobility assays whereas those that bind with low affinity only form one complex. The validity of the distinction between the two families of aptamers was confirmed by a functional in vivo deadenylation assay. Only those aptamers that bound CUG-BP1 with high affinity conferred deadenylation on a reporter mRNA. These high-affinity RNAs are characterized by a richness in UGU motifs. Using these binding site characteristics we identified the Xenopus maternal mRNA encoding the MAPK (mitogen-activated protein kinase) phosphatase (XCl100α) as a substrate for EDEN-BP. In conclusion, high-affinity CUG-BP1 binding sites are sequence elements at least 30 nucleotides in length that are enriched in combinations of U and G nucleotides and contain at least 4 UGU trinucleotide motifs. Such sequence elements are functionally competent to target an RNA for deadenylation in vivo.
Skip Nav Destination
Article navigation
December 2006
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
Research Article|
November 14 2006
CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding
Julien Marquis;
Julien Marquis
1
*Généthon, CNRS UMR 8115, 1 bis rue de l'Internationale 91002 Evry cedex 2, France
Search for other works by this author on:
Luc Paillard;
Luc Paillard
†CNRS UMR 6061, Génétique et Développement, IFR 140 GFAS, Université de Rennes 1, Faculté de Médecine, 2 Avenue Léon Bernard, CS 34317, 35043 Rennes Cedex, France
Search for other works by this author on:
Yann Audic;
Yann Audic
†CNRS UMR 6061, Génétique et Développement, IFR 140 GFAS, Université de Rennes 1, Faculté de Médecine, 2 Avenue Léon Bernard, CS 34317, 35043 Rennes Cedex, France
Search for other works by this author on:
Bertrand Cosson;
Bertrand Cosson
2
†CNRS UMR 6061, Génétique et Développement, IFR 140 GFAS, Université de Rennes 1, Faculté de Médecine, 2 Avenue Léon Bernard, CS 34317, 35043 Rennes Cedex, France
Search for other works by this author on:
Olivier Danos;
Olivier Danos
*Généthon, CNRS UMR 8115, 1 bis rue de l'Internationale 91002 Evry cedex 2, France
Search for other works by this author on:
Christine Le Bec;
Christine Le Bec
*Généthon, CNRS UMR 8115, 1 bis rue de l'Internationale 91002 Evry cedex 2, France
Search for other works by this author on:
H. Beverley Osborne
H. Beverley Osborne
3
†CNRS UMR 6061, Génétique et Développement, IFR 140 GFAS, Université de Rennes 1, Faculté de Médecine, 2 Avenue Léon Bernard, CS 34317, 35043 Rennes Cedex, France
3To whom correspondence should be addressed (email beverley.osborne@univ-rennes1.fr).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
April 03 2006
Revision Received:
July 25 2006
Accepted:
August 29 2006
Accepted Manuscript online:
August 29 2006
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2006
Biochem J (2006) 400 (2): 291–301.
Article history
Received:
April 03 2006
Revision Received:
July 25 2006
Accepted:
August 29 2006
Accepted Manuscript online:
August 29 2006
Citation
Julien Marquis, Luc Paillard, Yann Audic, Bertrand Cosson, Olivier Danos, Christine Le Bec, H. Beverley Osborne; CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding. Biochem J 1 December 2006; 400 (2): 291–301. doi: https://doi.org/10.1042/BJ20060490
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.