Two sPPases (soluble inorganic pyrophosphatases, EC 3.6.1.1) have been isolated from the microalga Chlamydomonas reinhardtii. Both are monomeric proteins of organellar localization, the chloroplastic sPPase I [Cr (Ch. reinhardtii)-sPPase I, 30 kDa] is a major isoform and slightly larger protein than the mitochondrial sPPase II (Cr-sPPase II, 24 kDa). They are members of sPPase family I and are encoded by two different cDNAs, as demonstrated by peptide mass fingerprint analysis. Molecular phylogenetic analyses indicated that Cr-sPPase I is closely related to other eukaryotic sPPases, whereas Cr-sPPase II resembles its prokaryotic counterparts. Chloroplastic sPPase I may have replaced a cyanobacterial ancestor very early during plastid evolution. Cr-sPPase II orthologues are found in members of the green photosynthetic lineage, but not in animals or fungi. These two sPPases from photosynthetic eukaryotes are novel monomeric family I sPPases with different molecular phylogenies and cellular localizations.
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Research Article|
March 15 2006
A novel subfamily of monomeric inorganic pyrophosphatases in photosynthetic eukaryotes
María R. Gómez-García;
1Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC-Universidad de Sevilla, Centro de Investigaciones Científicas Isla de la Cartuja, 41092-Sevilla, Spain
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Manuel Losada;
Manuel Losada
1Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC-Universidad de Sevilla, Centro de Investigaciones Científicas Isla de la Cartuja, 41092-Sevilla, Spain
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Aurelio Serrano
Aurelio Serrano
1
1Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC-Universidad de Sevilla, Centro de Investigaciones Científicas Isla de la Cartuja, 41092-Sevilla, Spain
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Publisher: Portland Press Ltd
Received:
October 11 2005
Revision Received:
November 22 2005
Accepted:
November 29 2005
Accepted Manuscript online:
November 29 2005
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2006
Biochem J (2006) 395 (1): 211–221.
Article history
Received:
October 11 2005
Revision Received:
November 22 2005
Accepted:
November 29 2005
Accepted Manuscript online:
November 29 2005
Citation
María R. Gómez-García, Manuel Losada, Aurelio Serrano; A novel subfamily of monomeric inorganic pyrophosphatases in photosynthetic eukaryotes. Biochem J 1 April 2006; 395 (1): 211–221. doi: https://doi.org/10.1042/BJ20051657
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