Ephrins are cell surface ligands that activate Eph receptor tyrosine kinases. This ligand–receptor interaction plays a central role in the sorting of cells. We have previously shown that the ephrinB–EphB signalling pathway is also involved in the migration of intestinal precursor cells along the crypts. Using the colon cell line DLD1 expressing the EphB2 receptor, we showed that stimulation of these cells with soluble ephrinB1 results in a rapid retraction of cell extensions and a detachment of cells. On ephrinB1 stimulation, the small GTPases Rho and Ras are activated and Rap1 is inactivated. Importantly, when a constitutively active Rap1 mutant was introduced into these cells, ephrinB1-induced retraction was inhibited. From these results, we conclude that down-regulation of Rap1 is a prerequisite for ephrin-induced cell retraction in colon cells.
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Research Article|
July 05 2005
Down-regulation of Rap1 activity is involved in ephrinB1-induced cell contraction
Jurgen A. Riedl;
Jurgen A. Riedl
1
*Department of Physiological Chemistry and Centre for Biomedical Genetics, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
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Dominique T. Brandt;
*Department of Physiological Chemistry and Centre for Biomedical Genetics, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
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Eduard Batlle;
Eduard Batlle
3
†Hubrecht Laboratory, Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
4To whom correspondence should be addressed (email j.l.bos@med.uu.nl).
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Leo S. Price;
Leo S. Price
*Department of Physiological Chemistry and Centre for Biomedical Genetics, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
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Hans Clevers;
Hans Clevers
†Hubrecht Laboratory, Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
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Johannes L. Bos
Johannes L. Bos
4
*Department of Physiological Chemistry and Centre for Biomedical Genetics, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
4To whom correspondence should be addressed (email j.l.bos@med.uu.nl).
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Publisher: Portland Press Ltd
Received:
January 06 2005
Revision Received:
January 21 2005
Accepted:
February 22 2005
Accepted Manuscript online:
February 22 2005
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2005
Biochem J (2005) 389 (2): 465–469.
Article history
Received:
January 06 2005
Revision Received:
January 21 2005
Accepted:
February 22 2005
Accepted Manuscript online:
February 22 2005
Citation
Jurgen A. Riedl, Dominique T. Brandt, Eduard Batlle, Leo S. Price, Hans Clevers, Johannes L. Bos; Down-regulation of Rap1 activity is involved in ephrinB1-induced cell contraction. Biochem J 15 July 2005; 389 (2): 465–469. doi: https://doi.org/10.1042/BJ20050048
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