Tetraspanins function as molecular organizers of multi-protein complexes by assembling primary complexes of a relatively low mass into extensive networks involved in cellular signalling. In this paper, we summarize our studies performed on the tetraspanin D6.1A/CO-029/TM4SF3 expressed by rat carcinoma cells. Primary complexes of D6.1A are almost indistinguishable from complexes isolated with anti-CD9 antibody. Indeed, both tetraspanins directly associate with each other and with a third tetraspanin, CD81. Moreover, FPRP (prostaglandin F2α receptor-regulatory protein)/EWI-F/CD9P-1), an Ig superfamily member that has been described to interact with CD9 and CD81, is also a prominent element in D6.1A complexes. Primary complexes isolated with D6.1A-specific antibody are clearly different from complexes containing the tetraspanin CD151. CD151 is found to interact only with D6.1A if milder conditions, i.e. lysis with LubrolWX instead of Brij96, are applied to disrupt cellular membranes. CD151 probably mediates the interaction of D6.1A primary complexes with α3β1 integrin. In addition, two other molecules were identified to be part of D6.1A complexes at this higher level of association: type II phosphatidylinositol 4-kinase and EpCAM, an epithelial marker protein overexpressed by many carcinomas. The characterization of the D6.1A core complex and additional more indirect interactions will help to elucidate the role in tumour progression and metastasis attributed to D6.1A.
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Research Article|
June 21 2005
The tetraspanin D6.1A and its molecular partners on rat carcinoma cells
Christoph CLAAS;
Christoph CLAAS
1
*Department of Tumor Progression and Immune Defense, Deutsches Krebsforschungszentrum, Heidelberg, Germany
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Joachim WAHL;
Joachim WAHL
1
*Department of Tumor Progression and Immune Defense, Deutsches Krebsforschungszentrum, Heidelberg, Germany
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David J. ORLICKY;
David J. ORLICKY
†Department of Pathology, University of Colorado Health Sciences Center, Denver, CO, U.S.A.
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Handan KARADUMAN;
Handan KARADUMAN
*Department of Tumor Progression and Immune Defense, Deutsches Krebsforschungszentrum, Heidelberg, Germany
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Martina SCHNÖLZER;
Martina SCHNÖLZER
‡Central Unit for Protein Analysis, Deutsches Krebsforschungszentrum, Heidelberg, Germany
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Tore KEMPF;
Tore KEMPF
‡Central Unit for Protein Analysis, Deutsches Krebsforschungszentrum, Heidelberg, Germany
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Margot ZÖLLER
Margot ZÖLLER
2
*Department of Tumor Progression and Immune Defense, Deutsches Krebsforschungszentrum, Heidelberg, Germany
§Department of Applied Genetics, University Karlsruhe, Germany
2To whom correspondence should be addressed (email m.zoeller@dkfz.de).
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Publisher: Portland Press Ltd
Received:
July 30 2004
Revision Received:
February 11 2005
Accepted:
February 22 2005
Accepted Manuscript online:
February 22 2005
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2005
Biochem J (2005) 389 (1): 99–110.
Article history
Received:
July 30 2004
Revision Received:
February 11 2005
Accepted:
February 22 2005
Accepted Manuscript online:
February 22 2005
Citation
Christoph CLAAS, Joachim WAHL, David J. ORLICKY, Handan KARADUMAN, Martina SCHNÖLZER, Tore KEMPF, Margot ZÖLLER; The tetraspanin D6.1A and its molecular partners on rat carcinoma cells. Biochem J 1 July 2005; 389 (1): 99–110. doi: https://doi.org/10.1042/BJ20041287
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