By applying in vivo dimethyl sulphate and UV light type C-footprinting analysis, we previously showed that specific DNA sequences in the −1349/+42 core promoter region of the inducible human BDKRB1 (bradykinin B1 receptor) gene correlated with its transcriptional activity. In the present study we used the highly sensitive DNase I in vivo footprinting approach to delineate more precisely the functional domains of the BDKRB1 gene promoter in human SMCs (smooth muscle cells). Human lymphocytes that do not express a functional BDKRB1 were also studied as a reference using dimethyl sulphate, UV light type C and DNase I treatments. An obvious difference was found in the DNase I-footprinting patterns between cellular systems that express a functional BDKRB1 (SMCs) in comparison with human lymphocytes, where randomly distributed nucleosome-like footprinting patterns were found in the bulk of the core promoter region studied. Gel-shift assays and expression studies pointed to the implication of the YY1 and a TBP/TFIIB (TATA-box-binding protein/transcription factor IIB) transcription factor in the regulation of BDKRB1 gene expression in SMCs and possible YY1 involvement in the mechanisms of nuclear factor κB-mediated regulation of the receptor expression. No significant changes in the promoter foot-printing pattern were found after treatment with interleukin-1β or serum (known BDKRB1 gene inducers), indicating that definite regulatory motifs could exist outside the BDKRB1 gene core promoter region studied.
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Research Article|
June 21 2005
In vivo DNase I-mediated footprinting analysis along the human bradykinin B1 receptor (BDKRB1) gene promoter: evidence for cell-specific regulation
Martin ANGERS;
Martin ANGERS
*Unité de Recherche en Génétique Humaine et Moléculaire, Research Centre, Hôpital St-François d'Assise, Centre Hospitalier Universitaire de Québec, 10 de l'Espinay Street, QC, Canada G1L 3L5
†Division of Pathology, Department of Medical Biology, Faculty of Medicine, Laval University, QC, Canada
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Régen DROUIN;
Régen DROUIN
1
*Unité de Recherche en Génétique Humaine et Moléculaire, Research Centre, Hôpital St-François d'Assise, Centre Hospitalier Universitaire de Québec, 10 de l'Espinay Street, QC, Canada G1L 3L5
†Division of Pathology, Department of Medical Biology, Faculty of Medicine, Laval University, QC, Canada
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Magdalena BACHVAROVA;
Magdalena BACHVAROVA
‡Cancer Research Centre, Hôpital l'Hôtel-Dieu de Québec, Centre Hospitalier Universitaire de Québec, 9 rue McMahon, QC, Canada G1R 2J6
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Isabelle PARADIS;
Isabelle PARADIS
1
*Unité de Recherche en Génétique Humaine et Moléculaire, Research Centre, Hôpital St-François d'Assise, Centre Hospitalier Universitaire de Québec, 10 de l'Espinay Street, QC, Canada G1L 3L5
†Division of Pathology, Department of Medical Biology, Faculty of Medicine, Laval University, QC, Canada
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Brad BISSELL;
Brad BISSELL
§Division of Endocrinology, Department of Medicine, Beth Israel Deaconess Medical Center Harvard Medical School, 99 Brookline Ave., RN313, Boston, MA 02215, U.S.A.
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Makoto HIROMURA;
Makoto HIROMURA
§Division of Endocrinology, Department of Medicine, Beth Israel Deaconess Medical Center Harvard Medical School, 99 Brookline Ave., RN313, Boston, MA 02215, U.S.A.
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Anny USHEVA;
Anny USHEVA
§Division of Endocrinology, Department of Medicine, Beth Israel Deaconess Medical Center Harvard Medical School, 99 Brookline Ave., RN313, Boston, MA 02215, U.S.A.
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Dimcho BACHVAROV
Dimcho BACHVAROV
2
‡Cancer Research Centre, Hôpital l'Hôtel-Dieu de Québec, Centre Hospitalier Universitaire de Québec, 9 rue McMahon, QC, Canada G1R 2J6
∥Department of Medicine, Faculty of Medicine, Laval University, QC, Canada
2To whom correspondence should be addressed, at Cancer Research Centre, Hôpital l'Hôtel-Dieu de Québec (email dimtcho.batchvarov@crhdq.ulaval.ca).
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Publisher: Portland Press Ltd
Received:
December 17 2004
Revision Received:
January 31 2005
Accepted:
February 11 2005
Accepted Manuscript online:
February 11 2005
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2005
Biochem J (2005) 389 (1): 37–46.
Article history
Received:
December 17 2004
Revision Received:
January 31 2005
Accepted:
February 11 2005
Accepted Manuscript online:
February 11 2005
Citation
Martin ANGERS, Régen DROUIN, Magdalena BACHVAROVA, Isabelle PARADIS, Brad BISSELL, Makoto HIROMURA, Anny USHEVA, Dimcho BACHVAROV; In vivo DNase I-mediated footprinting analysis along the human bradykinin B1 receptor (BDKRB1) gene promoter: evidence for cell-specific regulation. Biochem J 1 July 2005; 389 (1): 37–46. doi: https://doi.org/10.1042/BJ20042104
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