The widely held view that SLN (sarcolipin) would be the natural inhibitor of SERCA1 (sarcoplasmic/endoplasmic-reticulum Ca2+-ATPase 1), and PLB (phospholamban) its counterpart for SERCA2 inhibition is oversimplified and partially wrong. The expression of SLN and PLB mRNA and protein relative to SERCA1 or SERCA2 was assessed in ventricle, atrium, soleus and EDL (extensor digitorum longus) of mouse, rat, rabbit and pig. SLN protein levels were quantified by means of Western blotting using what appears to be the first successfully generated antibody directed against SLN. Our data confirm the co-expression of PLB and SERCA2a in cardiac muscle and the very low levels (in pig and rabbit) or the absence (in rat and mouse) of PLB protein in the slow skeletal muscle. In larger animals, the SLN mRNA and protein expression in the soleus and EDL correlates with SERCA1a expression, but, in rodents, SLN mRNA and protein show the highest abundance in the atria, which are devoid of SERCA1. In the rodent atria, SLN could therefore potentially interact with PLB and SERCA2a. No SLN was found in the ventricles of the different species studied, and there was no compensatory SLN up-regulation for the loss of PLB in PLB−/− mouse. In addition, we found that SLN expression was down-regulated at the mRNA and protein level in the atria of hypertrophic hearts of SERCA2b/b mice. These data suggest that superinhibition of SERCA by PLB-SLN complexes could occur in the atria of the smaller rodents, but not in those of larger animals.
Skip Nav Destination
Article navigation
July 2005
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
Research Article|
June 21 2005
Sarcolipin and phospholamban mRNA and protein expression in cardiac and skeletal muscle of different species
Peter VANGHELUWE;
Peter VANGHELUWE
*Laboratory of Physiology, O/N Gasthuisberg, K.U. Leuven, Herestraat 49, B-3000 Leuven, Belgium
Search for other works by this author on:
Marleen SCHUERMANS;
Marleen SCHUERMANS
*Laboratory of Physiology, O/N Gasthuisberg, K.U. Leuven, Herestraat 49, B-3000 Leuven, Belgium
Search for other works by this author on:
Ernö ZÁDOR;
Ernö ZÁDOR
†Institute of Biochemistry, Faculty of Medicine, University of Szeged, Dom ter 9, P.O. Box 427, H-6701 Szeged, Hungary
Search for other works by this author on:
Etienne WAELKENS;
Etienne WAELKENS
‡Laboratory of Biochemistry, O/N Gasthuisberg, K.U. Leuven, Herestraat 49, B-3000 Leuven, Belgium
Search for other works by this author on:
Luc RAEYMAEKERS;
Luc RAEYMAEKERS
*Laboratory of Physiology, O/N Gasthuisberg, K.U. Leuven, Herestraat 49, B-3000 Leuven, Belgium
Search for other works by this author on:
Frank WUYTACK
Frank WUYTACK
1
*Laboratory of Physiology, O/N Gasthuisberg, K.U. Leuven, Herestraat 49, B-3000 Leuven, Belgium
1To whom correspondence should be addressed (email frank.wuytack@med.kuleuven.ac.be).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
January 11 2005
Revision Received:
March 17 2005
Accepted:
March 31 2005
Accepted Manuscript online:
March 31 2005
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2005
Biochem J (2005) 389 (1): 151–159.
Article history
Received:
January 11 2005
Revision Received:
March 17 2005
Accepted:
March 31 2005
Accepted Manuscript online:
March 31 2005
Citation
Peter VANGHELUWE, Marleen SCHUERMANS, Ernö ZÁDOR, Etienne WAELKENS, Luc RAEYMAEKERS, Frank WUYTACK; Sarcolipin and phospholamban mRNA and protein expression in cardiac and skeletal muscle of different species. Biochem J 1 July 2005; 389 (1): 151–159. doi: https://doi.org/10.1042/BJ20050068
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.