The Shigella flexneri serotypes differ in the nature of their O-antigens. The addition of glucosyl or O-acetyl groups to the common backbone repeat units gives rise to the different serotypes. GtrII glucosylates rhamnose III of the O-antigen repeat unit, thus converting serotype Y (which has no modifications to the basic O-antigen repeat unit) into serotype 2a, the most prevalent serotype. In the present study, the topology of GtrII has been determined. GtrII has nine transmembrane helices, a re-entrant loop and three large periplasmic regions. Four critical residues (Glu40, Phe414, Cys435 and Lys478) were identified in two of the periplasmic regions. Despite the lack of sequence similarity between GtrII and the Gtrs from other serotypes, three of the critical residues identified are conserved in the remaining Gtrs. This is consistent with some degree of mechanistic conservation in this functionally related group of proteins.
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Research Article|
June 21 2005
Bacteriophage-encoded glucosyltransferase GtrII of Shigella flexneri: membrane topology and identification of critical residues
Adele M. LEHANE;
Adele M. LEHANE
1School of Biochemistry and Molecular Biology, Faculty of Science Building 41, The Australian National University, Canberra ACT 0200, Australia
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Haralambos KORRES;
Haralambos KORRES
1School of Biochemistry and Molecular Biology, Faculty of Science Building 41, The Australian National University, Canberra ACT 0200, Australia
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Naresh K. VERMA
Naresh K. VERMA
1
1School of Biochemistry and Molecular Biology, Faculty of Science Building 41, The Australian National University, Canberra ACT 0200, Australia
1To whom correspondence should be addressed (email Naresh.Verma@anu.edu.au).
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Publisher: Portland Press Ltd
Received:
January 14 2005
Revision Received:
March 03 2005
Accepted:
March 14 2005
Accepted Manuscript online:
March 14 2005
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2005
Biochem J (2005) 389 (1): 137–143.
Article history
Received:
January 14 2005
Revision Received:
March 03 2005
Accepted:
March 14 2005
Accepted Manuscript online:
March 14 2005
Citation
Adele M. LEHANE, Haralambos KORRES, Naresh K. VERMA; Bacteriophage-encoded glucosyltransferase GtrII of Shigella flexneri: membrane topology and identification of critical residues. Biochem J 1 July 2005; 389 (1): 137–143. doi: https://doi.org/10.1042/BJ20050102
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