Although several reports showed the effect of compounds disrupting microtubules on NF-κB (nuclear factor κB) activation, nothing is known about agents perturbing actin dynamics. In the present study, we have shown that actin cytoskeleton disruption induced by actin-depolymerizing agents such as cytochalasin D and latrunculin B and actin-polymerizing compounds such as jasplakinolide induced NF-κB activation in myelomonocytic cells. The transduction pathway involved the IκB (inhibitory κB) kinase complex and a degradation of IκBα. We have shown that NF-κB activation in response to the perturbation of actin dynamics required reactive oxygen species, as demonstrated by the effect of antioxidants. Actin cytoskeleton disruption by cytochalasin D induced O2− release from human monocytes, through the activation of the NADPH oxidase, as confirmed by the phosphorylation and by the membrane translocation of p47phox. NF-κB activation after actin cytoskeleton disruption could be physiologically relevant during monocyte activation and/or recruitment into injured tissues, where cellular attachment, migration and phagocytosis result in cyclic shifts in cytoskeletal organization and disorganization.
Skip Nav Destination
Article navigation
April 2005
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
Research Article|
April 05 2005
Perturbation of actin dynamics induces NF-κB activation in myelomonocytic cells through an NADPH oxidase-dependent pathway
Gaelle KUSTERMANS;
Gaelle KUSTERMANS
*Laboratory of Virology and Immunology, Institute of Pathology, University of Liège, B-4000 Liege, Belgium
Search for other works by this author on:
Jamel EL BENNA;
Jamel EL BENNA
†INSERM, Unité 479, Centre Hospitalier Universitaire Xavier Bichat, Paris, France
Search for other works by this author on:
Jacques PIETTE;
Jacques PIETTE
*Laboratory of Virology and Immunology, Institute of Pathology, University of Liège, B-4000 Liege, Belgium
Search for other works by this author on:
Sylvie LEGRAND-POELS
Sylvie LEGRAND-POELS
1
*Laboratory of Virology and Immunology, Institute of Pathology, University of Liège, B-4000 Liege, Belgium
1To whom correspondence should be addressed (email S.Legrand@ulg.ac.be).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
August 03 2004
Revision Received:
November 04 2004
Accepted:
November 09 2004
Accepted Manuscript online:
November 09 2004
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2005
Biochem J (2005) 387 (2): 531–540.
Article history
Received:
August 03 2004
Revision Received:
November 04 2004
Accepted:
November 09 2004
Accepted Manuscript online:
November 09 2004
Citation
Gaelle KUSTERMANS, Jamel EL BENNA, Jacques PIETTE, Sylvie LEGRAND-POELS; Perturbation of actin dynamics induces NF-κB activation in myelomonocytic cells through an NADPH oxidase-dependent pathway. Biochem J 15 April 2005; 387 (2): 531–540. doi: https://doi.org/10.1042/BJ20041318
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.