Mucins isolated from the stomach of Rhesus monkey are oligomeric glycoproteins with a similar mass, density, glycoform profile and tissue localization as human MUC5AC and MUC6. Antibodies raised against the human mucins recognize those from monkey, which thus appear to be orthologous to those from human beings. Rhesus monkey muc5ac and muc6 are produced by the gastric-surface epithelium and glands respectively, and occur as three distinct glycoforms. The mucins are substituted with the histo blood-group antigens B, Lea (Lewis a), Leb, Lex, Ley, H-type-2, the Tn-antigen, the T-antigen, the sialyl-Lex and sialyl-Lea structures, and the expression of these determinants varies between individuals. At neutral pH, Helicobacter pylori strains expressing BabA (blood-group antigen-binding adhesin) bind Rhesus monkey gastric mucins via the Leb or H-type-1 structures, apparently on muc5ac, as well as on a smaller putative mucin, and binding is inhibited by Leb or H-type-1 conjugates. A SabA (sialic acid-binding adhesin)-positive H. pylori mutant binds to sialyl-Lex-positive mucins to a smaller extent compared with the BabA-positive strains. At acidic pH, the microbe binds to mucins substituted by sialylated structures such as sialyl-Lex and sialylated type-2 core, and this binding is inhibited by DNA and dextran sulphate. Thus mucin–H. pylori binding occurs via at least three different mechanisms: (1) BabA-dependent binding to Leb and related structures, (2) SabA-dependent binding to sialyl-Lex and (3) binding through a charge-mediated mechanism to sialylated structures at low pH values.
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Research Article|
May 01 2004
Rhesus monkey gastric mucins: oligomeric structure, glycoforms and Helicobacter pylori binding1
Sara LINDÉN;
Sara LINDÉN
*Mucosal Biology Group, Department of Cell and Molecular Biology, Biomedical Center, Lund University, SE-22184 Lund, Sweden
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Thomas BORÉN;
Thomas BORÉN
†Department of Odontology/Oral Microbiology, Umeå University, SE-90187 Umeå, Sweden
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André DUBOIS;
André DUBOIS
‡Laboratory of Gastrointestinal and Liver Studies, Digestive Diseases Division, Department of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD, U.S.A.
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Ingemar CARLSTEDT
Ingemar CARLSTEDT
2
*Mucosal Biology Group, Department of Cell and Molecular Biology, Biomedical Center, Lund University, SE-22184 Lund, Sweden
2To whom correspondence should be addressed (e-mail Ingemar.Carlstedt@medkem.lu.se).
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Publisher: Portland Press Ltd
Received:
October 10 2003
Revision Received:
December 23 2003
Accepted:
January 15 2004
Accepted Manuscript online:
January 21 2004
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2004
2004
Biochem J (2004) 379 (3): 765–775.
Article history
Received:
October 10 2003
Revision Received:
December 23 2003
Accepted:
January 15 2004
Accepted Manuscript online:
January 21 2004
Citation
Sara LINDÉN, Thomas BORÉN, André DUBOIS, Ingemar CARLSTEDT; Rhesus monkey gastric mucins: oligomeric structure, glycoforms and Helicobacter pylori binding. Biochem J 1 May 2004; 379 (3): 765–775. doi: https://doi.org/10.1042/bj20031557
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