Type I PIPkins (phosphatidylinositol 4-phosphate 5-kinases) are the enzymes that catalyse the major cellular route of synthesis of PtdIns(4,5)P2, and three isoforms (α, β and γ) with several splice variants have been found to date. In the present paper, we describe the discovery of a novel splice variant of the γ isoform, which we call PIPkin Iγc, and which is characterized by the inclusion of a 26-amino-acid insert near the C-terminus. Its transcript appears to be selectively expressed in brain, where it locates in the neurons of restricted regions, such as cerebellum, hippocampus, cortex and olfactory bulb, as indicated by in situ hybridization studies. Overexpression of two different catalytically inactive constructs of PIPkin Iγc in rat cerebellar granule cells causes a progressive loss of their neuronal processes, whereas equivalent kinase-dead versions of PIPkin Iγa did not induce any such effect, suggesting the possible existence of a specific PtdIns(4,5)P2 pool synthesized by PIPkin Iγc, which is involved in the maintenance of some neuronal cellular processes.
Skip Nav Destination
Article navigation
Research Article|
April 15 2004
A novel neuronal-specific splice variant of Type I phosphatidylinositol 4-phosphate 5-kinase isoform gamma
Maria-Luisa GIUDICI;
Maria-Luisa GIUDICI
*Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, U.K.
†Department of Biomedical Sciences and Biotechnology, University of Brescia, via Valsabbina 19, 25123 Brescia, Italy
Search for other works by this author on:
Piers C. EMSON;
Piers C. EMSON
‡Department of Neurobiology, The Babraham Institute, Cambridge CB2 4AT, U.K.
Search for other works by this author on:
Robin F. IRVINE
Robin F. IRVINE
1
*Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, U.K.
1To whom correspondence should be addressed (e-mail rfi20@cam.ac.uk).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
September 12 2003
Revision Received:
December 19 2003
Accepted:
January 23 2004
Accepted Manuscript online:
January 23 2004
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2004
2004
Biochem J (2004) 379 (2): 489–496.
Article history
Received:
September 12 2003
Revision Received:
December 19 2003
Accepted:
January 23 2004
Accepted Manuscript online:
January 23 2004
Citation
Maria-Luisa GIUDICI, Piers C. EMSON, Robin F. IRVINE; A novel neuronal-specific splice variant of Type I phosphatidylinositol 4-phosphate 5-kinase isoform gamma. Biochem J 15 April 2004; 379 (2): 489–496. doi: https://doi.org/10.1042/bj20031394
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.