HDGF (hepatoma-derived growth factor) and the HRPs (HDGF-related proteins) comprise a family of six proteins which display high identity in their N-terminus, but differ at the C-terminus. Here we investigate the patterns of expression of HDGF and HRP-3, by generating antisera specifically recognizing each growth factor. Whereas HRP-3 protein is expressed only in brain, HDGF can be found in a broad range of tissues, with highest levels in brain, testis, lung and spleen. The expression of HDGF and HRP-3 was found to be regulated during brain development, with highest levels around birth, followed by a decline until postnatal day 9. Interestingly, expression of HRP-3 increases again in adult brain. In situ hybridization and immunohistochemistry of cerebellar, cerebral and hippocampal brain slices showed that expression of both growth factors is not limited to areas of high proliferative activity. Both mRNAs and proteins are expressed in neuronal as well as glial cells. Immunocytochemistry of cultured neocortical neurons revealed that HDGF and HRP-3 can be found in the nucleus as well as the cytoplasm. HDGF is restricted to the neuronal soma, whereas HRP-3 can also be found in neurites. Thus the expression of HDGF and HRP-3 in differentiated cells, post-mitotic neurons and primary cultures of rat neocortex points to functions in brain that might not be limited to proliferation. In addition, their simultaneous expression in the same cell and their different subcellular localization in cultured neurons suggest different functions of HDGF and HRP-3 within single cells.
Skip Nav Destination
Article navigation
Research Article|
February 15 2004
Expression patterns and different subcellular localization of the growth factors HDGF (hepatoma-derived growth factor) and HRP-3 (HDGF-related protein-3) suggest functions in addition to their mitogenic activity
Mekky M. ABOUZIED;
Mekky M. ABOUZIED
*Institut für Physiologische Chemie, Rheinische Friedrich-Wilhelms Universität, Nussallee 11, 53115 Bonn, Germany
Search for other works by this author on:
Stephan L. BAADER;
Stephan L. BAADER
†Anatomisches Institut, Anatomie und Zellbiologie, Rheinische Friedrich-Wilhelms Universität, Nussallee 10, 53115 Bonn, Germany
Search for other works by this author on:
Frank DIETZ;
Frank DIETZ
‡Institut für Physiologische Biochemie, Universität Bremen, Leobener Strasse NW2/B2, 28359 Bremen, Germany
Search for other works by this author on:
Joachim KAPPLER;
Joachim KAPPLER
*Institut für Physiologische Chemie, Rheinische Friedrich-Wilhelms Universität, Nussallee 11, 53115 Bonn, Germany
Search for other works by this author on:
Volkmar GIESELMANN;
Volkmar GIESELMANN
*Institut für Physiologische Chemie, Rheinische Friedrich-Wilhelms Universität, Nussallee 11, 53115 Bonn, Germany
Search for other works by this author on:
Sebastian FRANKEN
Sebastian FRANKEN
1
*Institut für Physiologische Chemie, Rheinische Friedrich-Wilhelms Universität, Nussallee 11, 53115 Bonn, Germany
1To whom correspondence should be addressed (e-mail franken@institut.physiochem.uni-bonn.de).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
June 19 2003
Revision Received:
October 01 2003
Accepted:
October 22 2003
Accepted Manuscript online:
October 22 2003
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2004
2004
Biochem J (2004) 378 (1): 169–176.
Article history
Received:
June 19 2003
Revision Received:
October 01 2003
Accepted:
October 22 2003
Accepted Manuscript online:
October 22 2003
Citation
Mekky M. ABOUZIED, Stephan L. BAADER, Frank DIETZ, Joachim KAPPLER, Volkmar GIESELMANN, Sebastian FRANKEN; Expression patterns and different subcellular localization of the growth factors HDGF (hepatoma-derived growth factor) and HRP-3 (HDGF-related protein-3) suggest functions in addition to their mitogenic activity. Biochem J 15 February 2004; 378 (1): 169–176. doi: https://doi.org/10.1042/bj20030916
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.