The protein tyrosine phosphatases (PTPs) PTP-SL, STEP and HePTP are mitogen-activated protein kinase (MAPK) substrates and regulators that bind to MAPKs through a kinase-interaction motif (KIM) located in their non-catalytic regulatory domains. We have found that the binding of these PTPs to the MAPKs extracellular-signal-regulated kinase 1 and 2 (ERK1/2), and p38α is differentially determined by the KIM-adjacent C-terminal regions of the PTPs, which have been termed kinase-specificity sequences, and is influenced by reducing agents. Under control conditions, PTP-SL bound preferentially to ERK1/2, whereas STEP and HePTP bound preferentially to p38α. Under reducing conditions, the association of p38α with STEP or HePTP was impaired, whereas the association with PTP-SL was unaffected. On the other hand, the association of ERK1/2 with HePTP was increased under reducing conditions, whereas the association with STEP or PTP-SL was unaffected. In intact cells, PTP-SL and STEP distinctively regulated the kinase activity and the nuclear translocation of ERK1/2 and p38α. Our results suggest that intracellular redox conditions could modulate the activity and subcellular location of ERK1/2 and p38α by controlling their association with their regulatory PTPs.
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May 15 2003
Differential interaction of the tyrosine phosphatases PTP-SL, STEP and HePTP with the mitogen-activated protein kinases ERK1/2 and p38alpha is determined by a kinase specificity sequence and influenced by reducing agents
Juan José MUÑOZ;
Juan José MUÑOZ
The Instituto de Investigaciones Citológicas, Amadeo de Saboya, 4, 46010 Valencia, Spain
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Céline TÁRREGA;
Céline TÁRREGA
The Instituto de Investigaciones Citológicas, Amadeo de Saboya, 4, 46010 Valencia, Spain
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Carmen BLANCO-APARICIO;
Carmen BLANCO-APARICIO
1
The Instituto de Investigaciones Citológicas, Amadeo de Saboya, 4, 46010 Valencia, Spain
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Rafael PULIDO
Rafael PULIDO
2
The Instituto de Investigaciones Citológicas, Amadeo de Saboya, 4, 46010 Valencia, Spain
2To whom correspondence should be addressed (e-mail rpulido@ochoa.fib.es).
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Publisher: Portland Press Ltd
Received:
December 16 2002
Revision Received:
February 12 2003
Accepted:
February 13 2003
Accepted Manuscript online:
February 13 2003
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2003
2003
Biochem J (2003) 372 (1): 193–201.
Article history
Received:
December 16 2002
Revision Received:
February 12 2003
Accepted:
February 13 2003
Accepted Manuscript online:
February 13 2003
Citation
Juan José MUÑOZ, Céline TÁRREGA, Carmen BLANCO-APARICIO, Rafael PULIDO; Differential interaction of the tyrosine phosphatases PTP-SL, STEP and HePTP with the mitogen-activated protein kinases ERK1/2 and p38alpha is determined by a kinase specificity sequence and influenced by reducing agents. Biochem J 15 May 2003; 372 (1): 193–201. doi: https://doi.org/10.1042/bj20021941
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