Type I interferons (IFNs) are cytokines that are used clinically as antiviral and antitumour agents. The interaction of IFNs with their heterodimeric type I IFN receptor comprised of IFNAR1 and IFNAR2 is a first step to inducing biological actions. Here, we describe the successful mimicry of IFN-β by a peptide isolated by phage-display screening using a neutralizing anti-IFN-β monoclonal antibody. The 15-mer peptide, designated SYR6, was shown to compete with IFN-β for binding to type I IFN receptor in a concentration-dependent manner, and was shown to elicit antiviral activity on cultured cells. This antiviral activity was not eliminated in the presence of neutralizing monoclonal antibodies to IFN-α, -β and -γ, and a low concentration of soluble type I IFN receptor, suggesting that it was not due to IFN contamination or the induction of endogenous IFNs by SYR6. This peptide might be a potent agonist to provide a mechanism of activating heterodimeric cytokine receptors.
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April 2003
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Research Article|
April 15 2003
A peptide mimetic of human interferon (IFN)-beta
Atsushi SATO;
Atsushi SATO
1
Pharmaceutical Research Laboratories, Toray Industries Inc., 1111, Tebiro, Kamakura, Kanagawa, 248-8555, Japan
1To whom correspondence should be addressed, at (present address) Laboratory for Developmental Gene Regulation, RIKEN Brain Science Institute, 2-1, Hirosawa, Wako, Saitama, 351-0198, Japan (e-mail atsato@brain.riken.go.jp).
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Saburo SONE
Saburo SONE
Pharmaceutical Research Laboratories, Toray Industries Inc., 1111, Tebiro, Kamakura, Kanagawa, 248-8555, Japan
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Publisher: Portland Press Ltd
Received:
June 26 2002
Revision Received:
December 19 2002
Accepted:
January 24 2003
Accepted Manuscript online:
January 24 2003
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2003
2003
Biochem J (2003) 371 (2): 603–608.
Article history
Received:
June 26 2002
Revision Received:
December 19 2002
Accepted:
January 24 2003
Accepted Manuscript online:
January 24 2003
Citation
Atsushi SATO, Saburo SONE; A peptide mimetic of human interferon (IFN)-beta. Biochem J 15 April 2003; 371 (2): 603–608. doi: https://doi.org/10.1042/bj20020993
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