Five markers of different kinds of oxidative damage to proteins [glutamic semialdehyde, aminoadipic semialdehyde, N∊-(carboxymethyl)lysine, N∊-(carboxyethyl)lysine and N∊-(malondialdehyde)lysine] and phospholipid fatty acyl composition were identified and measured in skeletal muscle mitochondria isolated from mice genetically engineered to underexpress or overexpress uncoupling protein 3 (UCP3). Mitochondria from UCP3-underexpressing mice had significantly higher levels of oxidative damage than wild-type controls, suggesting that UCP3 functions in vivo as part of the antioxidant defences of the cell, but mitochondria from UCP3-overexpressing mice had unaltered oxidative damage, suggesting that mild uncoupling in vivo beyond the normal basal uncoupling provides little protection against oxidative stress. Mitochondria from UCP3-underexpressing mice showed little change, but mitochondria from UCP3-overexpressing mice showed marked changes in mitochondrial phospholipid fatty acyl composition. These changes were very similar to those previously found to correlate with basal proton conductance in mitochondria from a range of species and treatments, suggesting that high protein expression, or some secondary result of uncoupling, may cause the observed correlation between basal proton conductance and phospholipid fatty acyl composition.
Skip Nav Destination
Article navigation
December 2002
- PDF Icon PDF LinkFront Matter
Research Article|
December 01 2002
Oxidative damage and phospholipid fatty acyl composition in skeletal muscle mitochondria from mice underexpressing or overexpressing uncoupling protein 3
Martin D. BRAND;
Martin D. BRAND
1
∗MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, U.K.
1To whom correspondence should be addressed (e-mail martin.brand@mrc-dunn.cam.ac.uk).
Search for other works by this author on:
Reinald PAMPLONA;
Reinald PAMPLONA
†Metabolic Physiopathology Research Group, Department of Basic Medical Sciences, Faculty of Medicine, Lleida University, Lleida 25198, Spain
Search for other works by this author on:
Manuel PORTERO-OTÍN;
Manuel PORTERO-OTÍN
†Metabolic Physiopathology Research Group, Department of Basic Medical Sciences, Faculty of Medicine, Lleida University, Lleida 25198, Spain
Search for other works by this author on:
Jesús R. REQUENA;
Jesús R. REQUENA
2
‡Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, U.S.A.,
Search for other works by this author on:
Stephen J. ROEBUCK;
Stephen J. ROEBUCK
∗MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, U.K.
Search for other works by this author on:
Julie A. BUCKINGHAM;
Julie A. BUCKINGHAM
∗MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, U.K.
Search for other works by this author on:
John C. CLAPHAM;
John C. CLAPHAM
3
§Department of Vascular Biology, SmithKline Beecham Pharmaceuticals, Harlow, Essex CM19 5AW, U.K.
Search for other works by this author on:
Susana CADENAS
Susana CADENAS
4
∗MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, U.K.
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
July 09 2002
Revision Received:
August 16 2002
Accepted:
August 23 2002
Accepted Manuscript online:
August 23 2002
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2002
2002
Biochem J (2002) 368 (2): 597–603.
Article history
Received:
July 09 2002
Revision Received:
August 16 2002
Accepted:
August 23 2002
Accepted Manuscript online:
August 23 2002
Citation
Martin D. BRAND, Reinald PAMPLONA, Manuel PORTERO-OTÍN, Jesús R. REQUENA, Stephen J. ROEBUCK, Julie A. BUCKINGHAM, John C. CLAPHAM, Susana CADENAS; Oxidative damage and phospholipid fatty acyl composition in skeletal muscle mitochondria from mice underexpressing or overexpressing uncoupling protein 3. Biochem J 1 December 2002; 368 (2): 597–603. doi: https://doi.org/10.1042/bj20021077
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.