Expression of the ephrin-A4 ligand, a family member of ligands binding the Eph receptor tyrosine kinases, is induced after an antigen–receptor stimulation of lymphocytes. To understand the transcription regulation of the ephrin-A4 gene, its promoter was identified and regulating elements were characterized. The ephrin-A4 promoter contains cis elements directing the cell-specific expression. By deletion studies, three specific regions, which were contributing to the transcription activity in lymphoid cells, were localized. In one of these regions, an inverted CCAAT box was identified and shown to bind the transcription activator nuclear factor-Y (NF-Y). The importance of NF-Y binding for the ephrin-A4 promoter activity is shown by a total abrogation of promoter activity after destruction of its binding site. NF-Y binding and activity are also crucially dependent on the integrity of the surrounding sequence. In addition, electrophoretic mobility-shift assay and serial-mutation analysis of the two remaining regulating regions revealed cis regulatory elements contributing to the transcription activity of the ephrin-A4 promoter.
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September 2002
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Research Article|
September 01 2002
Characterization of the human ephrin-A4 promoter
Else MUNTHE;
Else MUNTHE
1
Department of Immunology, The Norwegian Radium Hospital, Montebello, N-0310 Oslo, Norway
1To whom correspondence should be addressed (e-mail elsem@labmed.uio.no).
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Hans-Christian AASHEIM
Hans-Christian AASHEIM
Department of Immunology, The Norwegian Radium Hospital, Montebello, N-0310 Oslo, Norway
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Publisher: Portland Press Ltd
Received:
November 20 2001
Revision Received:
April 19 2002
Accepted:
May 24 2002
Accepted Manuscript online:
May 24 2002
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2002
2002
Biochem J (2002) 366 (2): 447–458.
Article history
Received:
November 20 2001
Revision Received:
April 19 2002
Accepted:
May 24 2002
Accepted Manuscript online:
May 24 2002
Citation
Else MUNTHE, Hans-Christian AASHEIM; Characterization of the human ephrin-A4 promoter. Biochem J 1 September 2002; 366 (2): 447–458. doi: https://doi.org/10.1042/bj20011693
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