Rho GTPases control a complex network of intracellular signalling pathways. Whereas progress has been made in identifying downstream signalling partners for these proteins, the characterization of Rho upstream regulatory guanine-nucleotide exchange factors (GEFs) has been hampered by a lack of suitable research tools. Here we use small interfering RNAs (siRNAs) to examine the cellular regulation of the RhoB GTPase, and show that RhoB is activated downstream of the epidermal-growth-factor receptor through the Vav2 exchange factor. These studies demonstrate that siRNAs are an ideal research tool for the assignment of Rho GEF function in vivo.
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Research Article| September 01 2002
Small interfering RNAs as a tool to assign Rho GTPase exchange-factor function in vivo
Harry MELLOR 1
Mammalian Cell Biology Laboratory, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, U.K.
1To whom correspondence should be addressed (email firstname.lastname@example.org).
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Publisher: Portland Press Ltd
Received: May 29 2002
Revision Received: July 04 2002
Accepted: July 12 2002
Accepted Manuscript online: July 12 2002
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2002
Alexandra GAMPEL, Harry MELLOR; Small interfering RNAs as a tool to assign Rho GTPase exchange-factor function in vivo. Biochem J 1 September 2002; 366 (2): 393–398. doi: https://doi.org/10.1042/bj20020844
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