The role of NO in macrophage iron turnover was studied in macrophages from inducible nitric oxide synthase (iNOS)-deficient mice. Interferon γ/lipopolysaccharide (IFNγ/LPS)-activated bone marrow-derived macrophages from iNOS-deficient mice, following phagocytosis of 59Fe-labelled transferrin—anti-transferrin immune complexes, showed reduced iron release compared with cells from wild-type iNOS littermates. Uptake of the complexes by macrophages was similar in iNOS-deficient and wild-type mice. Ferritin was up-regulated by IFNγ/LPS treatment, but NO exercised a modest opposing down-regulatory effect. No effect of iNOS deficiency was seen when iron was taken up from iron citrate, which enters via a non-phagocytic route. These results suggest that NO plays a key role in regulating iron turnover in macrophages acquiring iron by phagocytosis of erythrocytes or cell debris, and thus the supply to peripheral tissues, such as to the bone marrow for erythropoiesis.
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July 2002
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Research Article|
July 01 2002
Regulation of phagosomal iron release from murine macrophages by nitric oxide
Victoriano MULERO;
Victoriano MULERO
1
∗Department of Immunology and Bacteriology, Western Infirmary, University of Glasgow, Glasgow, U.K.
†Department of Cell Biology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain
1To whom correspondence should be addressed, at the Department of Cell Biology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain (e-mail vmulero@um.es).
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Xiao-qing WEI;
Xiao-qing WEI
∗Department of Immunology and Bacteriology, Western Infirmary, University of Glasgow, Glasgow, U.K.
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Foo Y. LIEW;
Foo Y. LIEW
∗Department of Immunology and Bacteriology, Western Infirmary, University of Glasgow, Glasgow, U.K.
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Jeremy H. BROCK
Jeremy H. BROCK
∗Department of Immunology and Bacteriology, Western Infirmary, University of Glasgow, Glasgow, U.K.
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Publisher: Portland Press Ltd
Received:
December 21 2001
Revision Received:
March 18 2002
Accepted:
April 18 2002
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2002
2002
Biochem J (2002) 365 (1): 127–132.
Article history
Received:
December 21 2001
Revision Received:
March 18 2002
Accepted:
April 18 2002
Citation
Victoriano MULERO, Xiao-qing WEI, Foo Y. LIEW, Jeremy H. BROCK; Regulation of phagosomal iron release from murine macrophages by nitric oxide. Biochem J 1 July 2002; 365 (1): 127–132. doi: https://doi.org/10.1042/bj20011875
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