Stimulation of endothelial cells with receptor agonists leads to an activation of endothelial nitric oxide synthase (eNOS) that only lasts for a short duration. A more prolonged effect, however, is observed in response to non-receptor agonists, such as Ca2+ ionophores or thapsigargin (TG). To investigate the molecular mechanisms underlying the rapid deactivation of eNOS after stimulation with receptor agonists, we measured the time courses of eNOS activation and intracellular free Ca2+ concentration ([Ca2+]i) in response to bradykinin (BK) and ATP. Incubation of porcine aortic endothelial cells with BK (1μM) in the presence of 3mM extracellular Ca2+ increased [Ca2+]i from 110 to 350nM and enhanced the rate of l-[3H]citrulline formation from 0.1 to 5fmol/min. In the absence of extracellular Ca2+, the BK-induced increase in [Ca2+]i was only marginal (from 30 to 110nM) and not sufficient to activate eNOS. When Ca2+ (final concentration 3mM) was added 10min after BK, [Ca2+]i increased to 330nM within 3min, but interestingly, formation of l-[3H]citrulline was not detectable. A similar phenomenon was observed with ATP, but not with Ca2+ ionophores or TG. This indicates that stimulation of endothelial cells with receptor agonists leads to desensitization of eNOS, which renders the enzyme insensitive to activation by subsequent increases in [Ca2+]i. However, when ATP was added to BK-pretreated cells or, conversely, BK to ATP-pretreated cells, activation of eNOS was comparable with that of untreated cells, suggesting that BK and ATP affect different pools of eNOS. The desensitization of eNOS was reversible, since removal of ATP or BK from the incubation buffer restored the response to the respective agonist within 20min. In addition to the transient Ca2+ signal, desensitization of eNOS may represent a further mechanism by which endothelial cells rapidly terminate receptor-dependent NO formation.
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June 2002
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Research Article|
June 15 2002
Desensitization of endothelial nitric oxide synthase by receptor agonists
Sabine WAGNER;
Sabine WAGNER
Institut für Pharmakologie und Toxikologie, Karl-Franzens-Universität Graz, Universitätsplatz 2, A-8010 Graz, Austria
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Klaus GROSCHNER;
Klaus GROSCHNER
Institut für Pharmakologie und Toxikologie, Karl-Franzens-Universität Graz, Universitätsplatz 2, A-8010 Graz, Austria
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Bernd MAYER;
Bernd MAYER
Institut für Pharmakologie und Toxikologie, Karl-Franzens-Universität Graz, Universitätsplatz 2, A-8010 Graz, Austria
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Kurt SCHMIDT
Kurt SCHMIDT
1
Institut für Pharmakologie und Toxikologie, Karl-Franzens-Universität Graz, Universitätsplatz 2, A-8010 Graz, Austria
1To whom correspondence should be addressed (e-mail kurt.schmidt@uni-graz.at).
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Publisher: Portland Press Ltd
Received:
August 10 2001
Revision Received:
February 05 2002
Accepted:
April 08 2002
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2002
2002
Biochem J (2002) 364 (3): 863–868.
Article history
Received:
August 10 2001
Revision Received:
February 05 2002
Accepted:
April 08 2002
Citation
Sabine WAGNER, Klaus GROSCHNER, Bernd MAYER, Kurt SCHMIDT; Desensitization of endothelial nitric oxide synthase by receptor agonists. Biochem J 15 June 2002; 364 (3): 863–868. doi: https://doi.org/10.1042/bj20011178
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