Incubation of Jurkat cells with 4,5,6,7-tetrabromobenzotriazole (TBB), a specific inhibitor of protein kinase CK2, induces dose-and time-dependent apoptosis as judged by several criteria. TBB-promoted apoptosis is preceded by inhibition of Ser/Thr phosphorylation of haematopoietic lineage cell-specific protein 1 (HS1) and is accompanied by caspase-dependent fragmentation of the same protein. Both effects are also observable if apoptosis is promoted by anti-Fas antibodies and by etoposide. Moreover, in vitro experiments show that HS1, once phosphorylated by CK2, becomes refractory to cleavage by caspase-3. These findings, in conjunction with similar data in the literature concerning two other CK2 protein substrates, Bid and Max, suggest that CK2 may play a general anti-apoptotic role through the generation of phosphorylated sites conferring resistance to caspase cleavage.
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Research Article|
May 08 2002
Protein kinase CK2 inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB) induces apoptosis and caspase-dependent degradation of haematopoietic lineage cell-specific protein 1 (HS1) in Jurkat cells
Maria RUZZENE;
Maria RUZZENE
∗Dipartimento di Chimica Biologica, Università di Padova, Viale G. Colombo, 3-35121 Padova, Italy
†Instituto Veneto di Medicina Molecolare, Via Orus 2, 35129 Padova, Italy
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Daniele PENZO;
Daniele PENZO
‡Dipartimento di Scienze Biomediche Sperimentali, Università di Padova, 35121 Padova, Italy
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Lorenzo A. PINNA
Lorenzo A. PINNA
1
∗Dipartimento di Chimica Biologica, Università di Padova, Viale G. Colombo, 3-35121 Padova, Italy
†Instituto Veneto di Medicina Molecolare, Via Orus 2, 35129 Padova, Italy
1To whom correspondence should be addressed (e-mail pinna@civ.bio.unipd.it).
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Publisher: Portland Press Ltd
Received:
November 30 2001
Revision Received:
January 30 2002
Accepted:
February 26 2002
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2002
2002
Biochem J (2002) 364 (1): 41–47.
Article history
Received:
November 30 2001
Revision Received:
January 30 2002
Accepted:
February 26 2002
Citation
Maria RUZZENE, Daniele PENZO, Lorenzo A. PINNA; Protein kinase CK2 inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB) induces apoptosis and caspase-dependent degradation of haematopoietic lineage cell-specific protein 1 (HS1) in Jurkat cells. Biochem J 15 May 2002; 364 (1): 41–47. doi: https://doi.org/10.1042/bj3640041
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