We have identified previously a new murine protein serine/threonine kinase, MPK38, closely related to the sucrose-non-fermenting protein kinase family [Gil, Yang, Lee, Choi and Ha (1997) Gene 195, 295–301]. Using the C-terminal half of the putative human counterpart of MPK38, HPK38, as a bait in a yeast two-hybrid screen of a human HeLa cDNA library, it was discovered that the zinc-finger-motif-containing protein, termed zinc-finger-like protein 9 (ZPR9), bound both HPK38 and MPK38. In a co-expression assay, ZPR9 associated with MPK38 in vivo, and we showed that the ZPR9 is also phosphorylated by MPK38. In addition, ZPR9 physically interacts with itself in mammalian cells. The ZPR9 cDNA hybridized with a mRNA species of approx. 1.7kb in Northern-blot analysis. The ZPR9 transcript was detected in all tissues examined, including lung, kidney, spleen, liver and brain. Co-expression of ZPR9 with MPK38 caused the accumulation of ZPR9 in the nucleus. These findings suggest a potentially important role for ZPR9 in MPK38-mediated signal transduction, and that ZPR9 is a physiological substrate of MPK38 in vivo.
Skip Nav Destination
Article navigation
February 2002
- PDF Icon PDF LinkFront Matter
Research Article|
January 25 2002
Phosphorylation of a novel zinc-finger-like protein, ZPR9, by murine protein serine/threonine kinase 38 (MPK38)
Hyun-A SEONG;
Hyun-A SEONG
∗Department of Biochemistry, School of Life Sciences, Research Center for Bioresource and Health, Chungbuk National University, 48 Gaeshin-dong, Cheongju, Chungbuk 361-763, Korea
Search for other works by this author on:
Minchan GIL;
Minchan GIL
1
∗Department of Biochemistry, School of Life Sciences, Research Center for Bioresource and Health, Chungbuk National University, 48 Gaeshin-dong, Cheongju, Chungbuk 361-763, Korea
Search for other works by this author on:
Kyong-Tai KIM;
Kyong-Tai KIM
†Department of Life Science, Pohang University of Science and Technology, San 31, Hyoja-dong, Pohang 790-784, Koreaand
Search for other works by this author on:
Sung-Jin KIM;
Sung-Jin KIM
‡Department of Pharmacology, School of Dentistry, Kyung Hee University, Haegi-dong, Seoul 130-701, Korea
Search for other works by this author on:
Hyunjung HA
Hyunjung HA
2
∗Department of Biochemistry, School of Life Sciences, Research Center for Bioresource and Health, Chungbuk National University, 48 Gaeshin-dong, Cheongju, Chungbuk 361-763, Korea
2To whom correspondence should be addressed (e-mail hyunha@cbucc.chungbuk.ac.kr).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
July 25 2001
Revision Received:
October 15 2001
Accepted:
November 12 2001
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2002
2002
Biochem J (2002) 361 (3): 597–604.
Article history
Received:
July 25 2001
Revision Received:
October 15 2001
Accepted:
November 12 2001
Citation
Hyun-A SEONG, Minchan GIL, Kyong-Tai KIM, Sung-Jin KIM, Hyunjung HA; Phosphorylation of a novel zinc-finger-like protein, ZPR9, by murine protein serine/threonine kinase 38 (MPK38). Biochem J 1 February 2002; 361 (3): 597–604. doi: https://doi.org/10.1042/bj3610597
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.