Apart from leucocyte–endothelial interactions, the adhesion molecule L-selectin mediates the homotypic adhesion of leucocytes during recruitment at sites of acute inflammation, as well as intercellular adhesion of haematopoietic progenitor cells during haematopoiesis. There is evidence that, in addition to P-selectin glycoprotein ligand-1, other as-yet-unidentified proteins function as L-selectin ligands on human leucocytes and haematopoietic progenitor cells. In the present study, we show: (i) by affinity chromatography on L-selectin–agarose; (ii) by protein identification using MS; and (iii) by covalent cell-surface labelling with sulphosuccinimidyl-2-(biotinamido)ethyl-1,3-dithiopropionate that the multifunctional nuclear protein nucleolin is partly exposed on the cell surface, and is a ligand of L-selectin in human leucocytes and haematopoietic progenitor cells.
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December 2001
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Research Article|
December 10 2001
Identification of nucleolin as a new L-selectin ligand
Gesche HARMS;
Gesche HARMS
∗Institut für Klinische Chemie und Pathobiochemie, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Hindenburgdamm 30, 12200 Berlin, Germany
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Regine KRAFT;
Regine KRAFT
†Max-Delbrueck Zentrum für Molekulare Medizin, Robert-Roessle-Str. 10, 13125 Berlin, Germany
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Gerlinde GRELLE;
Gerlinde GRELLE
†Max-Delbrueck Zentrum für Molekulare Medizin, Robert-Roessle-Str. 10, 13125 Berlin, Germany
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Bärbel VOLZ;
Bärbel VOLZ
∗Institut für Klinische Chemie und Pathobiochemie, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Hindenburgdamm 30, 12200 Berlin, Germany
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Jens DERNEDDE;
Jens DERNEDDE
∗Institut für Klinische Chemie und Pathobiochemie, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Hindenburgdamm 30, 12200 Berlin, Germany
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Rudolf TAUBER
Rudolf TAUBER
1
∗Institut für Klinische Chemie und Pathobiochemie, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Hindenburgdamm 30, 12200 Berlin, Germany
1To whom correspondence should be addressed (e-mail tauber@ukbf.fu-berlin.de).
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Publisher: Portland Press Ltd
Received:
October 18 2001
Accepted:
November 01 2001
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2001
2001
Biochem J (2001) 360 (3): 531–538.
Article history
Received:
October 18 2001
Accepted:
November 01 2001
Citation
Gesche HARMS, Regine KRAFT, Gerlinde GRELLE, Bärbel VOLZ, Jens DERNEDDE, Rudolf TAUBER; Identification of nucleolin as a new L-selectin ligand. Biochem J 15 December 2001; 360 (3): 531–538. doi: https://doi.org/10.1042/bj3600531
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