High-density lipoproteins (HDLs) are considered anti-atherogenic because they mediate peripheral cell cholesterol transport to the liver for excretion and degradation. An important step in this reverse cholesterol-transport pathway is the uptake of cellular cholesterol by a specific subclass of small, lipid-poor apolipoprotein A-I particles designated preβ-HDL. The two lipid-transfer proteins present in human plasma, cholesteryl ester transfer protein (CETP) and phospholipid transfer protein (PLTP), have both been implicated in the formation of preβ-HDL. In order to investigate the relative contribution of each of these proteins, we used transgenic mouse models. Comparisons were made between human CETP transgenic mice (huCETPtg), human PLTP transgenic mice (huPLTPtg) and mice transgenic for both lipid-transfer proteins (huCETPtg/huPLTPtg). These animals showed elevated plasma levels of CETP activity, PLTP activity or both activities, respectively. We evaluated the generation of preβ-HDL in mouse plasma by immunoblotting and crossed immuno-electrophoresis. Generation of preβ-HDL was equal in huCETPtg and wild-type mice. In contrast, in huPLTPtg and huCETPtg/huPLTPtg mice, preβ-HDL generation was 3-fold higher than in plasma from either wild-type or huCETPtg mice. Our findings demonstrate that, of the two plasma lipid-transfer proteins, PLTP rather than CETP is responsible for the generation of preβ-HDL. These data support the hypothesis of a role for PLTP in the initial stage of reverse cholesterol transport.
Skip Nav Destination
Article navigation
December 2001
- PDF Icon PDF LinkFront Matter
Research Article|
November 26 2001
Evaluation of phospholipid transfer protein and cholesteryl ester transfer protein as contributors to the generation of preβ-high-density lipoproteins
Jessica LIE;
Jessica LIE
1
∗Department of Biochemistry, Cardiovascular Research Institute COEUR, Erasmus University Rotterdam, P. O. Box 1738, 3000 DR Rotterdam, The Netherlands
Search for other works by this author on:
Rini de CROM;
Rini de CROM
1
†MGC-Department of Cell Biology and Genetics, Erasmus University Rotterdam, P. O. Box 1738, 3000 DR Rotterdam, The Netherlands
‡Department of Vascular Surgery, Academic Hospital ‘'Dijkzigt'’, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
Search for other works by this author on:
Matti JAUHIAINEN;
Matti JAUHIAINEN
§Department of Biochemistry, National Public Health Institute, Mannerheimintie 166, FIN-0300 Helsinki, Finland
Search for other works by this author on:
Teus van GENT;
Teus van GENT
∗Department of Biochemistry, Cardiovascular Research Institute COEUR, Erasmus University Rotterdam, P. O. Box 1738, 3000 DR Rotterdam, The Netherlands
Search for other works by this author on:
Rien van HAPEREN;
Rien van HAPEREN
†MGC-Department of Cell Biology and Genetics, Erasmus University Rotterdam, P. O. Box 1738, 3000 DR Rotterdam, The Netherlands
Search for other works by this author on:
Leo SCHEEK;
Leo SCHEEK
∗Department of Biochemistry, Cardiovascular Research Institute COEUR, Erasmus University Rotterdam, P. O. Box 1738, 3000 DR Rotterdam, The Netherlands
Search for other works by this author on:
Hans JANSEN;
Hans JANSEN
∗Department of Biochemistry, Cardiovascular Research Institute COEUR, Erasmus University Rotterdam, P. O. Box 1738, 3000 DR Rotterdam, The Netherlands
Search for other works by this author on:
Christian EHNHOLM;
Christian EHNHOLM
§Department of Biochemistry, National Public Health Institute, Mannerheimintie 166, FIN-0300 Helsinki, Finland
Search for other works by this author on:
Arie van TOL
Arie van TOL
2
∗Department of Biochemistry, Cardiovascular Research Institute COEUR, Erasmus University Rotterdam, P. O. Box 1738, 3000 DR Rotterdam, The Netherlands
2To whom correspondence should be addressed (e-mail vantol@bc1.fgg.eur.nl).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
June 11 2001
Revision Received:
August 20 2001
Accepted:
September 19 2001
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2001
2001
Biochem J (2001) 360 (2): 379–385.
Article history
Received:
June 11 2001
Revision Received:
August 20 2001
Accepted:
September 19 2001
Citation
Jessica LIE, Rini de CROM, Matti JAUHIAINEN, Teus van GENT, Rien van HAPEREN, Leo SCHEEK, Hans JANSEN, Christian EHNHOLM, Arie van TOL; Evaluation of phospholipid transfer protein and cholesteryl ester transfer protein as contributors to the generation of preβ-high-density lipoproteins. Biochem J 1 December 2001; 360 (2): 379–385. doi: https://doi.org/10.1042/bj3600379
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.