Both plasminogen (Pg) activation and matrix metalloproteinases (MMPs) are involved in the proteolytic degradation of extracellular matrix components, a requisite event for malignant cell metastasis. The highly invasive 1-LN human prostate tumour cell line synthesizes and secretes large amounts of Pg activators and MMPs. We demonstrate here that the Pg type 2 (Pg 2) receptor in these cells is composed primarily of the membrane glycoprotein dipeptidyl peptidase IV (DPP IV). Pg 2has six glycoforms that differ in their sialic acid content. Only the highly sialylated Pg 2γ, Pg 2δ and Pg 2ε glycoforms bind to DPP IV via their carbohydrate chains and induce a Ca2+ signalling cascade; however, Pg 2ε alone is also able to significantly stimulate expression of MMP-9. We further demonstrate that the Pg-mediated invasive activity of 1-LN cells is dependent on the availability of Pg 2ε. This is the first demonstration of a direct association between the expression of MMP-9 and the Pg activation system.
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April 2001
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Research Article|
April 06 2001
Interaction of plasminogen with dipeptidyl peptidase IV initiates a signal transduction mechanism which regulates expression of matrix metalloproteinase-9 by prostate cancer cells
Mario GONZALEZ-GRONOW;
Mario GONZALEZ-GRONOW
1
*Department of Pathology, Box 3712, Duke University Medical Center, Durham, NC 27710, U.S.A.
1To whom correspondence should be addressed (e-mail gonza002@mc.duke.edu).
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Hernan E. GRENETT;
Hernan E. GRENETT
†Department of Medicine, Division of Cardiovascular Diseases, University of Alabama, Birmingham, AL 35294, U.S.A.
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Michael R. WEBER;
Michael R. WEBER
*Department of Pathology, Box 3712, Duke University Medical Center, Durham, NC 27710, U.S.A.
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Govind GAWDI;
Govind GAWDI
*Department of Pathology, Box 3712, Duke University Medical Center, Durham, NC 27710, U.S.A.
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Salvatore V. PIZZO
Salvatore V. PIZZO
*Department of Pathology, Box 3712, Duke University Medical Center, Durham, NC 27710, U.S.A.
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Publisher: Portland Press Ltd
Received:
October 26 2000
Revision Received:
January 10 2001
Accepted:
February 07 2001
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 2001
2001
Biochem J (2001) 355 (2): 397–407.
Article history
Received:
October 26 2000
Revision Received:
January 10 2001
Accepted:
February 07 2001
Citation
Mario GONZALEZ-GRONOW, Hernan E. GRENETT, Michael R. WEBER, Govind GAWDI, Salvatore V. PIZZO; Interaction of plasminogen with dipeptidyl peptidase IV initiates a signal transduction mechanism which regulates expression of matrix metalloproteinase-9 by prostate cancer cells. Biochem J 15 April 2001; 355 (2): 397–407. doi: https://doi.org/10.1042/bj3550397
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