The core of adenylate and guanylate cyclases is formed by an intramolecular or intermolecular dimer of two cyclase domains arranged in an antiparallel fashion. Metazoan membrane-bound adenylate cyclases are composed of 12 transmembrane spanning regions, and two cyclase domains which function as a heterodimer and are activated by G-proteins. In contrast, membrane-bound guanylate cyclases have only one transmembrane spanning region and one cyclase domain, and are activated by extracellular ligands to form a homodimer. In the cellular slime mould, Dictyosteliumdiscoideum, membrane-bound guanylate cyclase activity is induced after cAMP stimulation; a G-protein-coupled cAMP receptor and G-proteins are essential for this activation. We have cloned a Dictyostelium gene, DdGCA, encoding a protein with 12 transmembrane spanning regions and two cyclase domains. Sequence alignment demonstrates that the two cyclase domains are transposed, relative to these domains in adenylate cyclases. DdGCA expressed in Dictyostelium exhibits high guanylate cyclase activity and no detectable adenylate cyclase activity. Deletion of the gene indicates that DdGCA is not essential for chemotaxis or osmo-regulation. The knock-out strain still exhibits substantial guanylate cyclase activity, demonstrating that Dictyostelium contains at least one other guanylate cyclase.
Skip Nav Destination
Article navigation
March 2001
-
Cover Image
Cover Image
- PDF Icon PDF LinkTable of Contents
Research Article|
March 08 2001
Guanylate cyclase in Dictyostelium discoideum with the topology of mammalian adenylate cyclase
Jeroen ROELOFS;
Jeroen ROELOFS
1Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747AG Groningen, The Netherlands
Search for other works by this author on:
Helena SNIPPE;
Helena SNIPPE
1Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747AG Groningen, The Netherlands
Search for other works by this author on:
Reinhard G. KLEINEIDAM;
Reinhard G. KLEINEIDAM
1Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747AG Groningen, The Netherlands
Search for other works by this author on:
Peter J.M. Van HAASTERT
Peter J.M. Van HAASTERT
1
1Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747AG Groningen, The Netherlands
1To whom correspondence should be addressed (e-mail P.J.M.Van.Haastert@chem.rug.nl).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
June 19 2000
Revision Received:
December 06 2000
Accepted:
January 12 2001
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 2001
2001
Biochem J (2001) 354 (3): 697–706.
Article history
Received:
June 19 2000
Revision Received:
December 06 2000
Accepted:
January 12 2001
Citation
Jeroen ROELOFS, Helena SNIPPE, Reinhard G. KLEINEIDAM, Peter J.M. Van HAASTERT; Guanylate cyclase in Dictyostelium discoideum with the topology of mammalian adenylate cyclase. Biochem J 15 March 2001; 354 (3): 697–706. doi: https://doi.org/10.1042/bj3540697
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.