Synaptotagmin (Syt) belongs to a family of type-I membrane proteins and is a protein that consists of a short extracellular N-terminus, a single transmembrane domain, two C2 domains and a short C-terminus. Here, we cloned and characterized a mouse orthologue of human KIAA1427 protein as an atypical Syt (named Syt XIII). Subcellular fractionation and antibody-uptake experiments indicate that Syt XIII is indeed a type-I membrane protein, but, unlike other Syt isoforms, lacks an N-terminal extracellular domain. Syt XIII C2 domains show relatively little similarity to Syt I (less than 35% identity at the amino acid level), and lack key amino acids responsible for Ca2+ binding. Because of these substitutions, the Syt XIII C2 domains did not show Ca2+-dependent phospholipid-binding activity, and Syt XIII is thus classified as a Ca2+-independent isoform. By contrast, the Syt XIII C-terminal domain is highly homologous with other Syt isoforms and can function as a common receptor for neurexin Iα in vitro. Since Syt XIII is expressed in various tissues outside the brain, Syt XIII may be involved in constitutive vesicle transport.
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March 2001
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Research Article|
February 22 2001
Characterization of KIAA1427 protein as an atypical synaptotagmin (Syt XIII)
Mitsunori FUKUDA;
Mitsunori FUKUDA
1
*Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
1To whom correspondence should be addressed (e-mail mnfukuda@brain.riken.go.jp).
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Katsuhiko MIKOSHIBA
Katsuhiko MIKOSHIBA
*Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
†Division of Molecular Neurobiology, Department of Basic Medical Science, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
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Publisher: Portland Press Ltd
Received:
September 25 2000
Revision Received:
November 30 2000
Accepted:
December 19 2000
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 2001
2001
Biochem J (2001) 354 (2): 249–257.
Article history
Received:
September 25 2000
Revision Received:
November 30 2000
Accepted:
December 19 2000
Citation
Mitsunori FUKUDA, Katsuhiko MIKOSHIBA; Characterization of KIAA1427 protein as an atypical synaptotagmin (Syt XIII). Biochem J 1 March 2001; 354 (2): 249–257. doi: https://doi.org/10.1042/bj3540249
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