An elevated cAMP concentration results in growth arrest and protein synthesis-dependent apoptosis in the promyelocytic leukaemia cell line IPC-81. A comparison of two-dimensional gels of extracts from these cells labelled with [35S]methionine revealed that five distinct protein spots were induced by cAMP in a protein-synthesis-dependent manner. The spots seemed to result from the acidic shift of a precursor protein. The most abundant spot was phospho-actin. The spots induced by cAMP in intact cells were induced by cAMP-dependent protein kinase (cAPK) during the translation in vitro of mRNA from the leukaemia cells. The effect of cAPK was strictly co-translational, none of the spots being induced when cAPK was added after translation. This suggested that the protein spots arose by co-translational phosphorylation catalysed by cAPK. Two of the protein spots, phospho-actin and a protein with a molecular mass of 30 kDa and an isoelectric point of 4.5, were studied further with respect to expression. They were produced during the whole pre-apoptotic period, had cellular half-lives of several hours and were induced by the same concentrations of cAMP analogue that induced apoptosis. It is suggested that the accumulation of co-translationally modified proteins could be important for long-term cAMP signalling.
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September 1999
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Research Article|
August 24 1999
cAMP induces co-translational modification of proteins in IPC-81 cells
Randi HOVLAND;
Randi HOVLAND
*Department of Anatomy and Cell Biology, University of Bergen, Årstadveien 19, N-5009 Bergen, Norway
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Anne P. DØSKELAND;
Anne P. DØSKELAND
†Department of Biochemistry and Molecular Biology, University of Bergen, Årstadveien 19, N-5009 Bergen, Norway
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Thor S. EIKHOM;
Thor S. EIKHOM
†Department of Biochemistry and Molecular Biology, University of Bergen, Årstadveien 19, N-5009 Bergen, Norway
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Bernard ROBAYE;
Bernard ROBAYE
‡Institute of Interdisciplinary Research, School of Medicine, Free University of Brussels, Route de Lennik 808, B-1070 Brussels, Belgium
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Stein O. DØSKELAND
Stein O. DØSKELAND
1
*Department of Anatomy and Cell Biology, University of Bergen, Årstadveien 19, N-5009 Bergen, Norway
1To whom correspondence should be addressed (stein.doskeland@pki.uib.no).
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Publisher: Portland Press Ltd
Received:
March 12 1999
Revision Received:
June 02 1999
Accepted:
June 22 1999
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1999
1999
Biochem J (1999) 342 (2): 369–377.
Article history
Received:
March 12 1999
Revision Received:
June 02 1999
Accepted:
June 22 1999
Citation
Randi HOVLAND, Anne P. DØSKELAND, Thor S. EIKHOM, Bernard ROBAYE, Stein O. DØSKELAND; cAMP induces co-translational modification of proteins in IPC-81 cells. Biochem J 1 September 1999; 342 (2): 369–377. doi: https://doi.org/10.1042/bj3420369
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