Doc2 is a C2-domain-containing protein that is highly expressed in the nervous system and has a constitutively expressed isoform. It has been implicated as a potential Ca2+ sensor in regulated exocytosis, and has been suggested to be associated with synaptic vesicles. To examine whether Doc2 is associated with synaptic-like microvesicles (SLMVs) or dense-core granules in neuroendocrine cells, we examined the distribution of Doc2 in subcellular fractionation of chromaffin cells of the adrenal medulla and in PC12 cells. Doc2 did not co-distribute with SLMVs from either cell type, but did appear to co-distribute with dense-core granules from PC12 cells. In contrast, it was not associated with the dense-core granules during subcellular fractionation of the adrenal medulla, and nor did it appear to be associated with endosomes, cis-Golgi or the trans-Golgi network. In contrast, Doc2 co-distributed under all conditions with a mitochondrial marker. We conclude that Doc2 is not a general component of regulated secretory vesicles, but may instead be associated with mitochondria.
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Research Article|
June 24 1999
Doc2 is not associated with known regulated exocytotic or endosomal compartments in adrenal chromaffin cells
Nathalie CHARVIN;
Nathalie CHARVIN
1The Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, U.K.
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Geoff WILLIAMS;
Geoff WILLIAMS
1The Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, U.K.
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Robert D. BURGOYNE
Robert D. BURGOYNE
1
1To whom correspondence should be addressed (e-mail burgoyne@liverpool.ac.uk).
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Publisher: Portland Press Ltd
Received:
February 02 1999
Revision Received:
April 06 1999
Accepted:
April 28 1999
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1999
1999
Biochem J (1999) 341 (1): 179–183.
Article history
Received:
February 02 1999
Revision Received:
April 06 1999
Accepted:
April 28 1999
Citation
Nathalie CHARVIN, Geoff WILLIAMS, Robert D. BURGOYNE; Doc2 is not associated with known regulated exocytotic or endosomal compartments in adrenal chromaffin cells. Biochem J 1 July 1999; 341 (1): 179–183. doi: https://doi.org/10.1042/bj3410179
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