Previous reports have demonstrated an increase in nuclear factor-κB (NF-κB) activity in response to UV radiation. These studies have essentially focused on the DNA-damaging fraction of solar UV radiation (UV-B and UV-C). In contrast, the effects of UV-A radiation (320–400 nm) on NF-κB are not well known. In this study, we present evidence that UV-A radiation induces a marked decrease in NF-κB DNA-binding activity in NCTC 2544 human keratinocytes. In addition, NCTC 2544 keratinocytes pretreated with UV-A fail to respond to NF-κB inducers. Moreover, UV-A radiation induces a decrease in NF-κB-driven luciferase reporter gene expression in NCTC 2544 keratinocytes. The expression of the gene encoding IκBα (IκB is the NF-κB inhibitor), which is closely associated with NF-κB activity, is also reduced (3-fold) upon UV-A treatment. Our results indicate that the UV-A-induced decrease in NF-κB DNA-binding activity is associated with a decrease in the levels of the p50 and p65 protein subunits. This is the first evidence that an oxidative stress, such as UV-A radiation, may induce a specific decrease in NF-κB activity in mammalian cells, probably through degradation of NF-κB protein subunits. These findings suggest that UV-A could modulate the NF-κB-dependent gene expression.
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March 1999
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Research Article|
March 08 1999
UV-A-induced decrease in nuclear factor-κB activity in human keratinocytes
Mojgan DJAVAHERI-MERGNY;
Mojgan DJAVAHERI-MERGNY
1
*Laboratoire de Dermatologie, INSERM U312, Hôpital Saint-Louis, 1 avenue Claude Vellefaux, 75475 Paris, France
1To whom correspondence should be sent, at present address: INSERM U365, Institut Curie, Recherche Section de Biologie, 26 Rue d'Ulm, 75005 Paris, France (e-mail Mojgan.Mergny@curie.fr).
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Marie-Pierre GRAS;
Marie-Pierre GRAS
*Laboratoire de Dermatologie, INSERM U312, Hôpital Saint-Louis, 1 avenue Claude Vellefaux, 75475 Paris, France
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Jean-Louis MERGNY;
Jean-Louis MERGNY
†Laboratoire de Biophysique, INSERM U201, CNRS UMR 8646, MNHN, 43 rue Cuvier, 75231 Paris Cedex 05, France
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Louis DUBERTRET
Louis DUBERTRET
*Laboratoire de Dermatologie, INSERM U312, Hôpital Saint-Louis, 1 avenue Claude Vellefaux, 75475 Paris, France
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Publisher: Portland Press Ltd
Received:
September 17 1998
Revision Received:
December 03 1998
Accepted:
December 22 1998
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1999
1999
Biochem J (1999) 338 (3): 607–613.
Article history
Received:
September 17 1998
Revision Received:
December 03 1998
Accepted:
December 22 1998
Citation
Mojgan DJAVAHERI-MERGNY, Marie-Pierre GRAS, Jean-Louis MERGNY, Louis DUBERTRET; UV-A-induced decrease in nuclear factor-κB activity in human keratinocytes. Biochem J 15 March 1999; 338 (3): 607–613. doi: https://doi.org/10.1042/bj3380607
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