In the yeast Saccharomyces cerevisiae, the non-Mendelian inherited genetic element [URE3] behaves as a prion. A hypothesis has been put forward which states that [URE3] arises spontaneously from its cellular isoform Ure2p (the product of the URE2 gene), and propagates through interactions of the N-terminal domain of the protein, thus leading to its aggregation and loss of function. In the present study, various N- and C-terminal deletion mutants of Ure2p were constructed and their cross-interactions were tested in vitro and in vivo using affinity binding and a two-hybrid analysis. We show that the self-interaction of the protein is mediated by at least two domains, corresponding to the first third of the protein (the so-called prion-forming domain) and the C-terminal catalytic domain.
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March 1999
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Research Article|
February 22 1999
Characterization of the interaction domains of Ure2p, a prion-like protein of yeast
Eric FERNANDEZ-BELLOT;
Eric FERNANDEZ-BELLOT
1Centre de Génétique Moléculaire du C.N.R.S., Laboratoire Propre Associé à l'Université Pierre-et-Marie-Curie, 91190 Gif-sur-Yvette, France
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Elisabeth GUILLEMET;
Elisabeth GUILLEMET
1Centre de Génétique Moléculaire du C.N.R.S., Laboratoire Propre Associé à l'Université Pierre-et-Marie-Curie, 91190 Gif-sur-Yvette, France
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Agnès BAUDIN-BAILLIEU;
Agnès BAUDIN-BAILLIEU
1Centre de Génétique Moléculaire du C.N.R.S., Laboratoire Propre Associé à l'Université Pierre-et-Marie-Curie, 91190 Gif-sur-Yvette, France
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Sébastien GAUMER;
Sébastien GAUMER
1Centre de Génétique Moléculaire du C.N.R.S., Laboratoire Propre Associé à l'Université Pierre-et-Marie-Curie, 91190 Gif-sur-Yvette, France
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Anton A. KOMAR;
Anton A. KOMAR
1Centre de Génétique Moléculaire du C.N.R.S., Laboratoire Propre Associé à l'Université Pierre-et-Marie-Curie, 91190 Gif-sur-Yvette, France
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Christophe CULLIN
Christophe CULLIN
1
1Centre de Génétique Moléculaire du C.N.R.S., Laboratoire Propre Associé à l'Université Pierre-et-Marie-Curie, 91190 Gif-sur-Yvette, France
1To whom correspondence should be addressed (e-mail cullin@cgm.cnrs-gif.fr).
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Biochem J (1999) 338 (2): 403–407.
Article history
Received:
September 10 1998
Revision Received:
November 06 1998
Accepted:
December 11 1998
Citation
Eric FERNANDEZ-BELLOT, Elisabeth GUILLEMET, Agnès BAUDIN-BAILLIEU, Sébastien GAUMER, Anton A. KOMAR, Christophe CULLIN; Characterization of the interaction domains of Ure2p, a prion-like protein of yeast. Biochem J 1 March 1999; 338 (2): 403–407. doi: https://doi.org/10.1042/bj3380403
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