Hepatocyte nuclear factor 1 was identified as the transcription factor binding to a 20 bp (-150 to -131) region of the gene for human dipeptidyl peptidase IV, which has been shown to be important for the expression of dipeptidyl peptidase IV in the human intestinal and hepatic epithelial cell lines Caco-2 and HepG2. Functional analysis of the hepatocyte nuclear factor 1 site was performed with two minimal dipeptidyl peptidase IV promoter constructs (-250 to -41, and -150 to -41) with and without a 3 bp mutation in the hepatocyte nuclear factor 1 sequence, and used in transient transfection experiments with Caco-2 cells. The results show that the mutated constructs were able to drive transcription at only 5–10% of the activity of the non-mutated controls. Co-transfection of 3T3 cells with hepatocyte nuclear factor 1 (α or β) and dipeptidyl peptidase IV promoter constructs (-250 to -41 or -150 to -41) resulted in a 2.5–6-fold increase in transcription over controls with hepatocyte nuclear factor 1α but not with hepatocyte nuclear factor 1β. The results of this study show that hepatocyte nuclear factor 1 binds to the -150 to -131 region of the human dipeptidyl peptidase IV promoter and is necessary for transcriptional activation of the gene for dipeptidyl peptidase IV.
Skip Nav Destination
Article navigation
February 1999
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Research Article|
February 08 1999
Regulation of the gene for human dipeptidyl peptidase IV by hepatocyte nuclear factor 1α
Roger H. ERICKSON;
Roger H. ERICKSON
1
*Gastrointestinal Research Laboratory (151-M2), Department of Veterans Affairs Medical Center, 4150 Clement Street, San Francisco, CA 94121, U.S.A.
†Department of Medicine, University of California, Third and Parnassus Avenues, San Francisco, CA 94143, U.S.A.
1To whom correspondence should be addressed (e-mail neko@itsa.ucsf.edu).
Search for other works by this author on:
James R. GUM;
James R. GUM
*Gastrointestinal Research Laboratory (151-M2), Department of Veterans Affairs Medical Center, 4150 Clement Street, San Francisco, CA 94121, U.S.A.
†Department of Medicine, University of California, Third and Parnassus Avenues, San Francisco, CA 94143, U.S.A.
Search for other works by this author on:
Craig D. LOTTERMAN;
Craig D. LOTTERMAN
*Gastrointestinal Research Laboratory (151-M2), Department of Veterans Affairs Medical Center, 4150 Clement Street, San Francisco, CA 94121, U.S.A.
†Department of Medicine, University of California, Third and Parnassus Avenues, San Francisco, CA 94143, U.S.A.
Search for other works by this author on:
James W. HICKS;
James W. HICKS
*Gastrointestinal Research Laboratory (151-M2), Department of Veterans Affairs Medical Center, 4150 Clement Street, San Francisco, CA 94121, U.S.A.
†Department of Medicine, University of California, Third and Parnassus Avenues, San Francisco, CA 94143, U.S.A.
Search for other works by this author on:
Roy S. LAI;
Roy S. LAI
*Gastrointestinal Research Laboratory (151-M2), Department of Veterans Affairs Medical Center, 4150 Clement Street, San Francisco, CA 94121, U.S.A.
†Department of Medicine, University of California, Third and Parnassus Avenues, San Francisco, CA 94143, U.S.A.
Search for other works by this author on:
Young S. KIM
Young S. KIM
*Gastrointestinal Research Laboratory (151-M2), Department of Veterans Affairs Medical Center, 4150 Clement Street, San Francisco, CA 94121, U.S.A.
†Department of Medicine, University of California, Third and Parnassus Avenues, San Francisco, CA 94143, U.S.A.
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
June 12 1998
Revision Received:
October 29 1998
Accepted:
November 24 1998
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1999
1999
Biochem J (1999) 338 (1): 91–97.
Article history
Received:
June 12 1998
Revision Received:
October 29 1998
Accepted:
November 24 1998
Citation
Roger H. ERICKSON, James R. GUM, Craig D. LOTTERMAN, James W. HICKS, Roy S. LAI, Young S. KIM; Regulation of the gene for human dipeptidyl peptidase IV by hepatocyte nuclear factor 1α. Biochem J 15 February 1999; 338 (1): 91–97. doi: https://doi.org/10.1042/bj3380091
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.