The topoisomerase II inhibitor etoposide induced apoptosis in HL-60 cells within 4 h of exposure to the drug, as measured by changes in morphology, DNA fragmentation and cytotoxicity assays. Etoposide-induced apoptosis was accompanied by an increase in polyamine efflux from the cells and a decrease in total polyamine content during the first 24 h of exposure to the drug. Although both enzyme activities increased slightly, there were no significant changes in spermidine/spermine N1-acetyltransferase activity or polyamine oxidase activity. After longer exposures (48–72 h), significant induction of spermidine/spermine N1-acetyltransferase activity and loss of polyamine content occurred. These results suggest that polyamine oxidation and the resultant hydrogen peroxide produced may be associated with the initiation of apoptosis, while induction of the acetyltransferase and overall loss of intracellular polyamines may be involved in the final, possibly necrotic, stages of cell death.
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January 1999
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Research Article|
December 17 1998
Changes in polyamine catabolism in HL-60 human promyelogenous leukaemic cells in response to etoposide-induced apoptosis
Gayle S. LINDSAY;
Gayle S. LINDSAY
1Departments of Medicine and Therapeutics and Biomedical Sciences, University of Aberdeen, Institute of Medical Sciences, Polwarth Building, Foresterhill, Aberdeen AB25 2ZD, Scotland, U.K.
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Heather M. WALLACE
Heather M. WALLACE
1
1Departments of Medicine and Therapeutics and Biomedical Sciences, University of Aberdeen, Institute of Medical Sciences, Polwarth Building, Foresterhill, Aberdeen AB25 2ZD, Scotland, U.K.
1To whom correspondence should be addressed (e-mail h.m.wallace@abdn.ac.uk).
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Publisher: Portland Press Ltd
Received:
July 23 1998
Revision Received:
September 01 1998
Accepted:
October 21 1998
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1999
1999
Biochem J (1999) 337 (1): 83–87.
Article history
Received:
July 23 1998
Revision Received:
September 01 1998
Accepted:
October 21 1998
Citation
Gayle S. LINDSAY, Heather M. WALLACE; Changes in polyamine catabolism in HL-60 human promyelogenous leukaemic cells in response to etoposide-induced apoptosis. Biochem J 1 January 1999; 337 (1): 83–87. doi: https://doi.org/10.1042/bj3370083
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