An Ets transcription factor family member, GETS-1, was cloned from a goldfish retina cDNA library. GETS-1 contains a conserved Ets DNA-binding domain at its N-terminus and is most similar to ternary complex factor (TCF) serum-response-factor protein-1a (SAP-1a). GETS-1 is expressed in many tissues, but is enriched in retina and brain. As with the TCFs SAP-1a and ets-related protein (ERP), overexpression of the GETS-1 promoter suppresses nicotinic acetylcholine receptor ε-subunit gene expression in cultured muscle cells. A consensus Ets binding site sequence in the promoter of the ε-subunit gene is required for GETS-1-mediated repression. GETS-1 repressor activity is abrogated by overexpression of an activated Ras/mitogen-activated protein kinase (MAP kinase) or by mutation of Ser-405, a MAP kinase phosphorylation site in GETS-1. Fusion proteins created between GETS-1 and the Gal4 DNA-binding domain show that, like other TCFs, GETS-1 contains a C-terminal activation domain that is activated by a Ras/MAP kinase signalling cascade. Interestingly, mutation of Ser-405 located within this activation domain abrogated transcriptional activation of the fusion protein.
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Research Article|
October 15 1998
Cloning and characterization of GETS-1, a goldfish Ets family member that functions as a transcriptional repressor in muscle
Daniel GOLDMAN;
Daniel GOLDMAN
1
*Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, U.S.A.
†Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, U.S.A.
1To whom correspondence should be addressed (e-mail neuroman@umich.edu).
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Mohan K. SAPRU;
Mohan K. SAPRU
*Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, U.S.A.
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Scott STEWART;
Scott STEWART
†Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, U.S.A.
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Joshua PLOTKIN;
Joshua PLOTKIN
†Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, U.S.A.
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Towia A. LIBERMANN;
Towia A. LIBERMANN
‡Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, U.S.A.
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Bohdan WASYLYK;
Bohdan WASYLYK
§Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS, INSERM, ULP, 1 rue Laurent Fries, BP 163, 67404 Illkirch cedex, France
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Kunliang GUAN
Kunliang GUAN
†Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, U.S.A.
ǁInstitute of Gerontology, University of Michigan, Ann Arbor, MI 48109, U.S.A.
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Publisher: Portland Press Ltd
Received:
April 16 1998
Revision Received:
July 03 1998
Accepted:
August 06 1998
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1998
1998
Biochem J (1998) 335 (2): 267–275.
Article history
Received:
April 16 1998
Revision Received:
July 03 1998
Accepted:
August 06 1998
Citation
Daniel GOLDMAN, Mohan K. SAPRU, Scott STEWART, Joshua PLOTKIN, Towia A. LIBERMANN, Bohdan WASYLYK, Kunliang GUAN; Cloning and characterization of GETS-1, a goldfish Ets family member that functions as a transcriptional repressor in muscle. Biochem J 15 October 1998; 335 (2): 267–275. doi: https://doi.org/10.1042/bj3350267
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