Expression of the type II membrane proteins of the rbAT/4F2hc family in Xenopus laevisoocytes results in the induction of amino acid transport activity. To elucidate the mechanism of action, amino acid transport was investigated in oocytes expressing the surface antigen 4F2hc. Leucine transport was mediated by a Na+-independent and a Na+-dependent transport mechanism. Both systems could be further discriminated by their stereochemical constraints. Isoleucine, with a branch at the β-position, shared only the Na+-independent transport system with leucine. Both transport systems were sensitive to inhibition by arginine, but only the Na+-independent system was sensitive to inhibition by 2-aminobicyclo[2,2,1]heptane-2-carboxylic acid. When compared with known transport systems the two transport activities could be described as similar to, but not identical with, mammalian systems b0,+ and y+L. The Na+-independent b0,+-like transport system was found both in rbAT and 4F2hc expressing oocytes, indicating that both proteins act in a similar way.
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August 1998
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Research Article|
August 01 1998
Discrimination of two amino acid transport activities in 4F2 heavy chain- expressing Xenopus laevis oocytes
Angelika BRÖER;
Angelika BRÖER
1Physiologisch-Chemisches Institut, Universität Tübingen, Hoppe-Seyler Strasse 4, 72076 Tübingen, Germany
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Bernd HAMPRECHT;
Bernd HAMPRECHT
1Physiologisch-Chemisches Institut, Universität Tübingen, Hoppe-Seyler Strasse 4, 72076 Tübingen, Germany
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Stefan BRÖER
Stefan BRÖER
1
1Physiologisch-Chemisches Institut, Universität Tübingen, Hoppe-Seyler Strasse 4, 72076 Tübingen, Germany
1To whom correspondence should be addressed (e-mail stefan.broeer@uni-tuebingen.de).
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Publisher: Portland Press Ltd
Received:
February 02 1998
Revision Received:
April 08 1998
Accepted:
April 17 1998
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1998
1998
Biochem J (1998) 333 (3): 549–554.
Article history
Received:
February 02 1998
Revision Received:
April 08 1998
Accepted:
April 17 1998
Citation
Angelika BRÖER, Bernd HAMPRECHT, Stefan BRÖER; Discrimination of two amino acid transport activities in 4F2 heavy chain- expressing Xenopus laevis oocytes. Biochem J 1 August 1998; 333 (3): 549–554. doi: https://doi.org/10.1042/bj3330549
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