Oxidative damage might be important in atherogenesis. Oxidized lipids are present at significant concentrations in advanced human plaque, although tissue antioxidants are mostly present at normal concentrations. Indirect evidence of protein modification (notably derivatization of lysine) or oxidation has been obtained by immunochemical methods; the specificities of these antibodies are unclear. Here we present chemical determinations of six protein-bound oxidation products: dopa, o-tyrosine, m-tyrosine, dityrosine, hydroxyleucine and hydroxyvaline, some of which reflect particularly oxy-radical-mediated reaction pathways, which seem to involve mainly the participation of transition- metal ions. We compared the relative abundance of these oxidation products in normal intima, and in human carotid plaque samples with that observed after radiolytically generated hydroxyl radical attack on BSA in vitro. The close similarities in relative abundances in the latter two circumstances indicate that hydroxyl radical damage might occur in plaque. The relatively higher level of dityrosine in plaque than that observed after radiolysis suggests the additional involvement of HOCl-mediated reactions in advanced plaque.
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August 1998
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Research Article|
August 01 1998
Evidence for roles of radicals in protein oxidation in advanced human atherosclerotic plaque
Shanlin FU;
Shanlin FU
*Cell Biology Group, The Heart Research Institute, 145 Missenden Road, Camperdown, NSW, 2050, Australia
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Michael J. DAVIES;
Michael J. DAVIES
†EPR Group, The Heart Research Institute, 145 Missenden Road, Camperdown, NSW, 2050, Australia
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Roland STOCKER;
Roland STOCKER
‡Biochemistry Group, The Heart Research Institute, 145 Missenden Road, Camperdown, NSW, 2050, Australia
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Roger T. DEAN
Roger T. DEAN
1
*Cell Biology Group, The Heart Research Institute, 145 Missenden Road, Camperdown, NSW, 2050, Australia
1To whom correspondence should be addressed (e-mail r.dean@hri.edu.au).
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Publisher: Portland Press Ltd
Received:
February 26 1998
Revision Received:
April 16 1998
Accepted:
April 23 1998
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1998
1998
Biochem J (1998) 333 (3): 519–525.
Article history
Received:
February 26 1998
Revision Received:
April 16 1998
Accepted:
April 23 1998
Citation
Shanlin FU, Michael J. DAVIES, Roland STOCKER, Roger T. DEAN; Evidence for roles of radicals in protein oxidation in advanced human atherosclerotic plaque. Biochem J 1 August 1998; 333 (3): 519–525. doi: https://doi.org/10.1042/bj3330519
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